对于基因芯片实验的动力学范围和结果可重复性的研究

杂交动力学和实验结果的可重复性是基因芯片研究中十分重要的内容，以单基因模拟芯片对DNA芯片的杂交动力学进行了初步的研究：将不同含量的植物cDNA点在玻片上制成DNA芯片，利用体外转录的方法得到植物基因的mRNA，然后通过反转录方法把荧光标记到cDNA上，用不同含量的荧光标记cDNA对芯片进行杂交，根据结果分析了靶基因和探针含量变化对芯片杂交信号产生的影响，利用多基因模拟芯片分析了不同基因的杂交动力学特点；利用12800点的高密度芯片进行了2组不同的表达谱实验，对芯片结果的可重复性做了验证。

Study on the Dynamics Range and Result Reproducibility of DNA Microarray Experiments

Dynamics range and result reproducibility are two important issues in the research of DNA microarray. The hybridization dynamics of microarray experiments is assessedusing microarrays spotted with serial concentrations of the target gene and hybridized with serial contents of probes. The influences of the concentrations of target genes and probes on the hybridization signals are analyzed based on the results. The hybridization character of different genes immobilized on the same microarray is analyzed. The result reproducibility of microarray experiments is validated through two groups of gene expression profiling experiments.