| 生物鉴定和喂养试验证明GNA赋予转基因水稻纯系对褐飞虱的抗性 |
| |
| 引用本文： | 唐克轩,孙小芬,万丙良,戚华雄,卢兴桂.生物鉴定和喂养试验证明GNA赋予转基因水稻纯系对褐飞虱的抗性[J].复旦学报(自然科学版),2001,40(1):108-111,116. |
| |
| 作者姓名： | 唐克轩 孙小芬 万丙良 戚华雄 卢兴桂 |
| |
| 作者单位： | 1. 复旦大学 生命科学学院
2. 湖北农科院杂交水稻研究中心, |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，教育部科学技术研究项目，101-01-01-01，R F97001594，， |
| |
| 摘 要： | 参照Rao等（1998）的褐飞虱生物鉴定和喂养方法，用水稻褐飞虱生物Ⅰ型的－龄若虫食喂，用基因枪法获得2个转基因水稻纯系。这2个纯系均含有并表达潮霉素抗性基因（hpt),gusA报告基因和雪花莲凝集素基因（gna)。褐飞虱生物鉴定和喂养试验表明，水稻纯系对褐飞虱具有显著的抑制作用。具体表现为降低褐飞虱成活率和繁殖力、延缓褐虱发育以及减少褐飞虱进食是。通过褐飞虱生物鉴定和喂养试验证明，表达GNA的转基因水稻纯系对严重危害水稻生产的褐飞虱具有抗性作用。
|
| 关 键 词： | 水稻 gna 转基因水稻 生物鉴定 喂养试验 褐飞虱 抗性 |
| 文章编号： | 0427-7104(2001)01-0108-04 |
GNA Confers Resistance to Rice Brown Planthopper in Transgenic Rice Pure Lines by Both Bioassay and Feeding Tests |
| |
| TANG Ke-Xuan,SUN Xiao-Fen,WAN Bing-liang,QI Hua-xing,LU Xing-gui.GNA Confers Resistance to Rice Brown Planthopper in Transgenic Rice Pure Lines by Both Bioassay and Feeding Tests[J].Journal of Fudan University(Natural Science),2001,40(1):108-111,116. |
| |
| Authors: | TANG Ke-Xuan SUN Xiao-Fen WAN Bing-liang QI Hua-xing LU Xing-gui |
| |
| Abstract: | Two transgenic rice pure lines, generated by particle bombardment, were challeng ed by the first-instar nymphs of rice brown planthopper (Nilaparvata lugens, BPH) biotype I using the standard BPH bioassay and feeding test protocol essen tially the same as described by Rao et al. (1998). Both lines contained and expressed the selectable marker hygromycin phosphotransferase gene(hpt), the reporter β-glucuronidase gene(gusA) and the snowdrop (Galanthus niv alis) lectin gene (gna). Insect bioassay and feeding test results showed t hat both lines had significant deterrent effect on BPH by decreasing BPH surviva l and overall fecundity, retarding BPH development and declining BPH feeding. |
| |
| Keywords: | Oryza sativa L gna transgenic rice bioassay feeding test |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
