| 50例中国人IX因子基因的限制性片段长度多态性 |
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| 引用本文： | 罗峰,秦世真,蒋左庶,邱信芳,薛京伦.50例中国人IX因子基因的限制性片段长度多态性[J].复旦学报(自然科学版),1990(1). |
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| 作者姓名： | 罗峰 秦世真 蒋左庶 邱信芳 薛京伦 |
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| 作者单位： | 复旦大学遗传学研究所
(罗峰，秦世真，邱信芳)，第二军医大学
(蒋左庶)，复旦大学遗传学研究所(薛京伦) |
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| 摘 要： | 用IX因子基因内探针F9(VⅢ)对TaqI,BamHI和EcoRI酶切的50例中国人基因组DNA进行杂交分析。结果表明,所有个体经TaqI酶切的杂交片段为4.5kb和1.8kb,BamHI和EcoRI酶切的杂交片段分别为23kb和5.0 kb。基因组DNA样本中未发现限制性片段长度多态性(RFLP),这与欧美国家的民族群体中存在着IX因子基因内TaqI和BamHI的RFLP的结论不同。造成不同种族间DNA水平差异的原因,很可能与长期在不同地理环境中的进化适应有关。
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| 关 键 词： | 人 多态现象 乙型血友病 中国人 IX因子 限制性片段长度多态性 |
STUDY ON THE RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)OF FACTOR IX GENE IN CHINESE POPULATION |
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| Luo Feng,Qin Shizhen,Jiang Zuoshu,Qiu Xinfang,Xue Jinglun,.STUDY ON THE RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)OF FACTOR IX GENE IN CHINESE POPULATION[J].Journal of Fudan University(Natural Science),1990(1). |
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| Authors: | Luo Feng Qin Shizhen Jiang Zuoshu Qiu Xinfang Xue Jinglun |
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| Institution: | Institute of Genetics |
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| Abstract: | The present study involves with RFLP analysis of factor IX gene in Chinesepopulation. The results obtained from Southern analysis of genomic DNA with F9(VIII) in 50 Chinese individuals were as follows: (1) Two 4.5 kb and 1.8 kb frag-ments were observed in all samples of genomic DNA digested with Taq I; (2) A23 kb BamH I fragment and a 5.0 kb EcoR I nonpolymorphic fragment was seenin samples of genomic DNA digested with BamH I and EcoR I, respectively.The differences in DNA sequences among different human races might reflectconstraints (environmental, geographical etc) pressed on the process of evolutionwas assumed. |
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| Keywords: | human polymorphism hemophilia B Ohinese factor IX restriction fragment length polymorphism |
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