NIH3T3细胞与其癌变细胞的PARP酶被抑制后DNA损伤修复的差异研究

PARP酶 (聚ADP核糖基聚合酶Poly ADP ribosepolymerase ,PARP)对于DNA损伤修复具有重要功能 ,正常细胞及其癌变细胞的该酶对抑制剂的敏感程度可能会有差异 .为此 ,通过姐妹染色体交换频率 ,带报告基因的质粒转化频率 ,以及彗星测定等方法 ,对DNA的损伤修复进行初步的检测 .实验结果表明 ,正常细胞和癌变细胞PARP酶在抑制剂作用下酶活性都会降低 ,但是癌变细胞的PARP酶对抑制剂更为敏感 .

Study on the Difference of DNA Damage and Repair Between the Normal Cell and Its Carcinomatous Cell After Treatment with PARP's Inhibitor

Poly(ADP ribose) polymerase (PARP) is a nuclear protein that catalyzes the formation of extensive branched polymers of poly(ADP ribose) attached to a protein acceptor using nicotinamide adenine dinucleotide (NAD +) as substrate. PARP responds rapidly to DNA damage and is thus important for DNA repair. In carcinomatous cells, this enzyme may show different sensitivity to inhibitor treatment as in normal cells. To test this hypothesis, the writer detected the DNA damage and repair effeciency in both carcinomatous and normal cells by means of SCE, transfection efficiency, Comet Assay after treatment with PARP inhibitor. In both normal and carcinomatous cells, the activity of this enzyme decreased under the inhibitor treatment, however, PARP in carcinomatous cells is more sensitive to inhibitor.