| 分子筛固定化葡萄糖淀粉酶的制备 |
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| 引用本文： | 于家波,夏春镗.分子筛固定化葡萄糖淀粉酶的制备[J].复旦学报(自然科学版),1999,38(6):644-647,651. |
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| 作者姓名： | 于家波 夏春镗 |
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| 作者单位： | 复旦大学(于家波，陈海鹰，胡仁其，李全芝)，上海铁道大学(夏春镗) |
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| 基金项目： | 国家自然科学基金!29673015 |
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| 摘 要： | 以改性分子筛作为固定化酶的载体，用固定化葡萄糖淀粉酶生产葡萄糖。一种新的基于多次吸附多次戊二醛交联的固定化方法增加了固定化葡萄糖淀粉酶的稳定性，另讨论了该方法的机理。制备的固定化葡萄糖淀粉酶在柱反应器中水解淀粉连续使用２２ｄ，产物ＤＥ值维持在９５左右基本保持不变。
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| 关 键 词： | 改性 分子筛 葡萄糖淀粉酶 固定化方法 制备 |
Preparation of Glucoamylase Immobilized to Molecular Sieve |
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| YU Jia-bo, CHEN Hai-ying, HU Ren-qi, XIA Chun-tang, LI Quan-zhi.Preparation of Glucoamylase Immobilized to Molecular Sieve[J].Journal of Fudan University(Natural Science),1999,38(6):644-647,651. |
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| Authors: | YU Jia-bo CHEN Hai-ying HU Ren-qi XIA Chun-tang LI Quan-zhi |
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| Institution: | 1. Fudan university; 2. Shanghai Tiedao university |
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| Abstract: | The modified molecular sieve were taken as support for the immobilization of glucoamylase (E. C. 3.2. 1. 3) to produce glucose. A new immobilization technique based on multiple times adsorbing and cross-linking was developed to improve the stability of the immobilized enzyme and the mechanism of immobilization was discussed. The immobilized enzyme prepared via this technique continuously hydrolyzes starch in a column reacter for 20 days keeping DE value about 95. |
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| Keywords: | modified molecular sieve glucoamylase immobilization techniquel multiple times adsorbing and cross-linking |
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