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棉花类耐盐锌指蛋白基因的克隆与结构分析
引用本文:王东,杨金水.棉花类耐盐锌指蛋白基因的克隆与结构分析[J].复旦学报(自然科学版),2002,41(1):42-46.
作者姓名:王东  杨金水
作者单位:1. 复旦大学生命科学学院遗传学研究所,上海200433;南京农业大学作物遗传育种系,南京,210095
2. 复旦大学生命科学学院遗传学研究所,上海,200433
摘    要:从棉花花瓣cDNA文库中随机挑选部分克隆,经测序发现一个与拟南芥耐盐锌指蛋白基因同源的cDNA(CSTZ),CSTZ序列全长1012bp,开放阅读框共编码272个氨基酸,含典型的植物双锌指(Cys2/His2)结构区,Northern杂交证实,CSTZ的表达随棉花幼苗钠盐处理浓度的升高而增强,在棉花花龄期,CSTZ基因在叶片,根,花瓣和花药组织中大量表达,在柱头组织中表达相对较弱。

关 键 词:棉花  耐盐  锌指蛋白  基因表达
文章编号:0427-7104(2002)01-0042-05

Cloning and Characterization of cDNA Encoding Cotton STZ-like Protein
WANG Dong ,YANG Jin shui.Cloning and Characterization of cDNA Encoding Cotton STZ-like Protein[J].Journal of Fudan University(Natural Science),2002,41(1):42-46.
Authors:WANG Dong    YANG Jin shui
Institution:WANG Dong 1,2,YANG Jin shui 1
Abstract:By sequencing analysis of the clones chosen from the cotton petal cDNA library, a cDNA, homologous to Arabidopsis salt tolerance zinc finger protein, was isolated and designated as CSTZ. This new cDNA (1 012 bp) contains an open reading frame of 819 bp encoding a 272 residue polypeptide that includes a typical plant two fingered Cys2/His2 zinc finger mitif. Nortern blot analysis revealed that the expressed level of CSTZ in cotton seedling increased in response to 220 mmol/L NaCl treatment. CSTZ is highly expressed in leaves, roots, petals, and anthers, but with low expression in stigmas.
Keywords:cotton  salt tolerance  zinc finger protein  gene expression
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