| 石蒜凝集素基因的克隆 |
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| 引用本文： | 姚剑虹,孙小芬,唐克轩.石蒜凝集素基因的克隆[J].复旦学报(自然科学版),2002,41(1):102-105. |
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| 作者姓名： | 姚剑虹 孙小芬 唐克轩 |
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| 作者单位： | 复旦大学生命科学学院遗传工程国家重点实验室,上海,200433 |
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| 基金项目： | 上海市科委资助项目，China National Transgenic Plant Research and Commercialization Special Project，Sino-UK Science and Technology Collaboration Fund，98JC14035，J99-A-007，，，， |
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| 摘 要： | 用石蒜科植物凝集素基因的保守序列为引物，利用RACE－PCR技术从石蒜幼嫩叶片中克隆出石蒜凝集素的全长cDNA，通过比较石蒜同其他石蒜科植物凝集素基因序列和推测的氨基酸序列，发现石蒜凝集素基因编码一具有信号肽的前体蛋白，石蒜凝集素同其他石蒜科植物凝集素一样为具有3个甘露糖专一结合盒的凝集素。
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| 关 键 词： | 石蒜科 cDNA 凝集素 石蒜 RACE－PCR |
Molecular Cloning of Lectin Gene from Lycoris radiata |
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| Abstract.Molecular Cloning of Lectin Gene from Lycoris radiata[J].Journal of Fudan University(Natural Science),2002,41(1):102-105. |
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| Authors: | Abstract |
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| Abstract: | By the use of RACE-PCR technique with RNA extracted from Lycoris radiata young leaves as template andprimers specifically designed according to conservative sequences of Amaryllidaceae lectins, the full-length cDNA of Ly-coris radiata lectin was cloned. Through comparative analysis of Lycoris radiata lectin gene and its deduced amino acidsequence with those of other Amaryllidaceae species,it was found that Lycoris radiata lectin gene encoded a precursorlectin with signal peptide. Lycoris radiata lectin was a mannose-binding lectin with three mannose-binding boxes likelectins from other Amaryllidaceae species. |
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| Keywords: | Amaryllidaceae cDNA lectin Lycoris radiata RACE PCR |
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