| 同源模建法建立和检验人类新基因ZNF191锌指基序的三维构象 |
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| 引用本文： | 毕安定,王顺德.同源模建法建立和检验人类新基因ZNF191锌指基序的三维构象[J].复旦学报(自然科学版),1998,37(2):123-128. |
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| 作者姓名： | 毕安定 王顺德 |
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| 基金项目： | 国家自然科学基金， 上海市科技发展基金 |
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| 摘 要： | 以实验室自行克隆的新基因ＺＮＦ１９１顺序为材料，按三联体密友转换出锌指基因编码的４个锌指结构域ＮＦ１９１ａ，ｂ，ｃ和ｄ的蛋白质一级顺序，并将它们与已用晶体衍射法确证了空间构象的７个Ｃｙｓ２Ｈｉｓ２（Ｃ２Ｈ２）型锌指进行同源比较，从中发现锌指７ＺＮＦ和１ＺＮＦ的一级结构与待检验的锌指顺序同源高且间隙较小。
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| 关 键 词： | 锌指蛋白 同源模建 QUANTA软件包 ZNF191 基因 |
Construction and Examination of the Confromation of ZNF191 Zinc-Finger Motifs Using Homology-Based Modeling |
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| Bi Anding, Wang Shunde, Wang Xiaoke, Yu Long,Shi Shaolin, Zhang Qi,Zhao Shouyuan.Construction and Examination of the Confromation of ZNF191 Zinc-Finger Motifs Using Homology-Based Modeling[J].Journal of Fudan University(Natural Science),1998,37(2):123-128. |
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| Authors: | Bi Anding Wang Shunde Wang Xiaoke Yu Long Shi Shaolin Zhang Qi Zhao Shouyuan |
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| Institution: | State Key Laboratory of Genetic Engineering |
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| Abstract: | Three dimension conformation of four putative zinc-finger motifs coded by a novel gene ZNF191 cloned in our laboratory was constructed and examined by using the homologybased modeling system. Data of 15 known zinc-finger motifs, 7 of which are C2H2 type,in PDB protein database were selected and used to compare with linear sequence of the zinc-finger motifs to be tested. An alignment was made between the sequence of four putative zinc-finger motifes and that of seven C2H2-type motifs seperately. Using QUANTA software package, each con formation relating to putative zinc-finger motifs was constructed and then examined by 3D profile method. It is shown that all of the putative zinc-finger motifs can form a zincfinger conformation properly. |
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| Keywords: | zinc-finger protein homology-based modeling QUANTA software 3D profile |
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