| 一个犬凝集素基因VIP36的人类同源基因cDNA的克隆、染色体定位和表达谱分析 |
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| 引用本文： | 汪玮,曾立,刘建平,唐榕,顾少华,方国洪,谢毅,毛裕民.一个犬凝集素基因VIP36的人类同源基因cDNA的克隆、染色体定位和表达谱分析[J].复旦学报(自然科学版),2000,39(6):588-592. |
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| 作者姓名： | 汪玮 曾立 刘建平 唐榕 顾少华 方国洪 谢毅 毛裕民 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,上海,200433 |
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| 基金项目： | 国家自然科学基金资助项目!(39870 40 2 ) |
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| 摘 要： | 从人的胎脑cDNA文库中克隆到一条犬凝集素基因VIP36的人类同源基因，此cDNA序列全长2430bp，拟编码一个382个氨基酸残基的蛋白，它编码的蛋白与犬VIP36有52％的同源性，因此将其命名为人类VIP36L基因，应用辐射杂交方法，钭该基因定位在人2号染色体的分子标记D2S388和D2S113之间，采用基因芯片杂交的方法研究其表达谱情况，发现该基因在胎皮和肝癌组织中表达量较高。
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| 关 键 词： | VIP36L基因 囊泡结合蛋白 辐射杂交 基因芯片 凝集素基因表达谱 克隆 |
Cloning, Mapping and Expression Analysis of a Human Homologous cDNA of Dog Lectin Gene, VIP 36 |
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| WANG Wei,ZENG Li,LIU Jian-ping,TANG Rong,GU Shao-hua,FANG Guo-hong,XIE Yi,MAO Yu-min.Cloning, Mapping and Expression Analysis of a Human Homologous cDNA of Dog Lectin Gene, VIP 36[J].Journal of Fudan University(Natural Science),2000,39(6):588-592. |
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| Authors: | WANG Wei ZENG Li LIU Jian-ping TANG Rong GU Shao-hua FANG Guo-hong XIE Yi MAO Yu-min |
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| Abstract: | A human homolog of dog lectin gene, VIP 36, was cloned from human fetal brain cDNA library. The 2 430 bp cDNA encodes a protein with 382 amino acids. The putative protein shows 52 % identity to VIP36 protein, so this gene is termed human VIP 36 L in agreement with the HUGO Nomenclature Committee. By using radiation hybrid mapping, the VIP 36 L gene is localized to human chromosome 2 between molecular markers D2S388 and D2S113. The relative tissue distribution of the VIP 36 L mRNA was determined by DNA microarray. From the results, the VIP 36 L mRNA was found in human fetal skin and liver cancer at higher level. |
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| Keywords: | VIP 36 L VIP36 (vesicular integral membrane protein) radiation hybrid (RH) DNA microarray |
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