灰飞虱共生菌Wolbachia引起的细胞质不亲和性

对我国６个地区和日本３个地区灰飞虱的Ｗｏｌｂａｈｉａ感染率，用ＰＣＲ技术进行了检测，结果表明中国的辽宁、北京、上海和云南的灰飞虱的Ｗｏｌｂａｃｈｉａ感染率均接近１００％；四川为５９．６％；而宁夏为０。各地区灰飞虱间的交配实验证明了在Ｗｏｌｂａｃｈｉａ引起的细胞质不亲和现象的存在，Ｗｏｌｂａｃｈｉａ１６ＳｒＤＮＡ的部分测序分析表明，上海、云南和日本出云的灰飞虱发的同为Ｗｏｌｂａｃｈｉａ ｐｉｐｉｅｎ

Cytoplasmic incompatibility caused by Wolbachia in small brown planthopper,Laodelphax striatellus Fallen

Wolbachia is a group of endosymbionts that habor diverse insect species. These microorganisms are maternally inherited and cause developmental defects of their hosts, including a phenomenon called cytoplasmic incompatibility (CI). In this paper, Wolbachia infections in the small brown planthopper, Laodelphax striatellus Fallen,from six locations in China and three from Japan were studied. The results had shown that the infection rates of Wolbachia are 100% for planthoppers from Liaoning, Beijing, Shanghai, and Yunnan,59. 6% for that of Sichuan, and none in Ningxia. The presence of CI was confirmed by mating experiments with planthopper populations from the above-mentioned locations in China.Sequencing of Wolbachia 16S rDNA for planthopers from Shanghai, Yunnan, and Izumo (Japan ) indicated that these Wolbachia strains harboring different planthopper populations belong to the same species,ie., Wolbachia pipientis.