人和小鼠神经干细胞的体外培养的分化研究

首次克隆了小鼠神经元标志性微管蛋白βⅢ基因，从核苷酸序列推导出小鼠与人两者之间在其羧基端有相同的EAQGPK六肽，进一步证实用抗人微管蛋白βⅢ单抗可检测小鼠神经干细胞分化成的神经元细胞，免疫组化鉴定显示小鼠神经干细胞在体积分数为1％胎牛血清（FBS）诱导下，可分化成神经元，星形胶质细胞，少突胶质细胞，同时培养了13周龄胎儿脑来源的人类神经干细胞，用特异性的抗人nestin抗体鉴定，全部为阳性细胞，但它们经诱导分化产生较不同寻常的细胞分化细胞和分化程度，在生长因子减半和1％FBS诱导条件下可分化为神经元和星形胶质细胞，而无少突胶质细胞分化，NF单抗检测证实为早期分化的神经元。

Culture and Differentiation of Human and Mouse Neural Stem Cells in vitro

A mouse neuron specific gene tubulin beta 3 has been cloned for the first time. It is found that there is the same epitope composed of six amino acids at the carboxyl terminal between human and mouse protein which was deduced from the nucleotide sequence of mouse tubulin beta 3. Thus, it is confirmed that mouse derived neurons can be detected with mouse anti human tubulin beta-3 monoclonal antibody.Mouse neural stem cells can differentiate into neurons, astrocytes and oligodendrocytes in vitro under the induction of 1% fetal bovine serum (FBS). Meanwhile, human neural stem cells from 13 week gestation forebrain tissue have been cultured successfully, all neural stem cells are positive stained by human specific anti nestin polyclonal antibody. When induced, they show the unusual results. With 1% FBS and half dose of two growth factors(EGF and bFGF), they can differentiate into neurons and astrocytes, but no oligodendrocytes. Further, the results of anti neurofilament monoclonal antibody assay show the differentiated neurons still stay in the early developing stage.