一种纯化茶皂素的方法

为了克服现有技术的缺陷,提高茶皂素的纯度和质量,扩展其应用范围,将市售的粗茶皂素配制成5%的水溶液,采用国产聚苯乙烯型树脂在pH=4进行吸附处理。0.1%氢氧化钠水溶液脱除被吸附的杂质和色素,以80%的甲醇为洗脱液,减压蒸馏回收甲醇,将残留物浓缩烘干,得到纯度95.1%、泡沫的罗氏迈尔高度达181 mm,溶血指数为1∶75000的产品。此茶皂素的纯化方法所用树脂选择性强,吸附量大,解吸和吸附容易,操作简便,成本低廉,生产周期短,有重要的实际应用价值。

THE METHOD OF PURIFICATION FOR TEA SAPONIN

In order to overcome the present defects in technology, enhance the purity and quality of tea saponin, enlarge its application range, 5% aqueous solution of crude tea saponin is prepared and then is adsorbed by polystyrene resin under pH=4. 0.1%NaOH aqueous solution is used to remove the impurities and coloring matter which are adsorbed, and 80%(v/v) methanol solution as the eluant of the tae saponin. The produced tea saponin has the purity 95. 1%, Ross-Miles foam height 181mm, and haemolysis index 1∶75000. The resin used in this purification has strong selectivity, large adsorbance, easy ofdesorption and adsorption, simple operation, low cost and short period of production. It is valuable for practical application.