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| 不同方法修饰玻璃表面制备蛋白质芯片的对比 | |
| 引用本文： | 蒋犁,余章斌,张春秀,唐祖明,陆祖宏,蒋涛.不同方法修饰玻璃表面制备蛋白质芯片的对比[J].东南大学学报(自然科学版),2007,37(1):136-140. |
| 作者姓名： | 蒋犁 余章斌 张春秀 唐祖明 陆祖宏 蒋涛 |
| 作者单位： | 1. 东南大学临床医学院,南京,210009 2. 东南大学临床医学院,南京,210009;南京市妇幼保健院,南京,210004 3. 东南大学生物电子学国家重点实验室,南京,210096 4. 南京市妇幼保健院,南京,210004 |
| 摘 要： | 分别采用3-氨基丙基-三甲氧基硅烷、戊二醛、多聚赖氨酸、聚丙烯酰胺、琼脂糖、硝化纤维修饰玻璃表面,对6种不同修饰表面制备的蛋白质芯片进行对比研究,探讨制备蛋白质芯片的合适玻璃表面修饰方法.利用原子力学显微镜对其表面进行表征,通过点样蛋白于修饰后的玻片表面制备蛋白质芯片,比较不同修饰表面对蛋白质的固定效率和固定效果.结果发现:氨基、醛基、聚赖氨酸修饰玻片为二维平面结构,琼脂糖、聚丙烯酰胺、硝化纤维修饰玻片其表面不仅有多孔结构,而且有一定厚度;琼脂糖修饰玻片所得的荧光强度最高,背景较低,是一种理想的制备蛋白质芯片的表面修饰方法. |
| 关 键 词： | 蛋白质芯片 氨基修饰 戊二醛修饰 聚赖氨酸修饰 聚丙烯酰胺修饰 琼脂糖修饰 |
| 文章编号： | 1001-0505（2007）01-0136-05 |
| 修稿时间： | 2006-04-07 |
Comparison between different modificative strategies of glass surfaces to prepare protein microarrays |
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| Jiang Li,Yu Zhangbin,Zhang Chunxiu,Tang Zuming,Lu Zuhong,Jiang Tao.Comparison between different modificative strategies of glass surfaces to prepare protein microarrays[J].Journal of Southeast University(Natural Science Edition),2007,37(1):136-140. | |
| Authors: | Jiang Li Yu Zhangbin Zhang Chunxiu Tang Zuming Lu Zuhong Jiang Tao |
| Institution: | 1 School of Clinical Medicine, Southeast University, Nanjing 210009, China;2 State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;3Nanjing Maternity and Child Health Hospital, Nanjing 210004, China |
| Abstract: | Glass slides were modified with 3-aminopropyltriethoxysilane,glutaraldehyde,poly-L-lysine,polyacrylamide,agarose film,and nitrocellulose to prepare protein microarrays.Atomic force microscopy was used to obtain three-dimensional surface profiles of the modified glass surfaces.The efficiency and the effects of immobilized proteins on the glass were compared.The results indicate that aminosilane,glutaraldehyde,and poly-L-lysine coated glass slides show two-dimensional surfaces;while glass slides modified with polyacrylamide,agarose,and nitrocellulose possess more holes and greater thickness.Agarose film coated glass slides demonstrate highest immobilization capacity,and lowest background,which is more suitable for preparing protein microarray. |
| Keywords: | protein microarray aminosilane modification glutaraldehyde modification poly-L-lysine modification polyacrylamide modification agarose modification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |