专性厌氧菌发酵罐制氢工艺

采用批式发酵工艺研究了专性厌氧菌P的产氢特性.分别探讨了P菌在小样及发酵罐扩大试验中的生长周期、pH值、葡萄糖浓度以及糖蜜浓度等生态因子对产氢能力的影响.结果表明,P菌是一种高效产氢的菌株,在培养10h后进入对数生长期和高速产氢阶段.当葡萄糖浓度为10 g/L,初始pH为7.0,接种比例为10％时,小样发酵和发酵罐的气体总产量均达到最大值,分别为485 mL和17.4L；在发酵罐中,通过连续补加NaOH溶液使pH恒定在7.0左右,可使每升培养基产氢量达到2.473 L,比小样提高了5％,此时葡萄糖分解率达到95.2％,且氢气的质量分数达到68.73％；利用糖蜜作为发酵底物,具有广阔的经济效益和除废产能的环境效益,当糖蜜底物浓度为35 g/L时的产气能力最佳,发酵罐中最大产气量为22 L.

Hydrogen production process of obligate anaerobic bacteria in fermentation tank

The characteristics of hydrogen production by obligate anaerobic strains P are studied with the method of batch culture.The effects of several ecological factors on hydrogen production ability are investigated in the sample and the fermentation tank,such as the growth cycle of bacteria P,the pH value,glucose concentration and molasses concentration.The results show that obligate anaerobic bacteria P is an efficient hydrogen production strain.After 10 h of growth,bacteria P comes into the logarithm growth period and the high-speed hydrogen production stage.When the conditions are controlled as glucose concentration of 10g/L,initial pH of 7.0 and vaccination ratio of 10%,the gas production yield of the sample and the fermentation tank both reach the maximum,i.e.485mL and 17.4 L,respectively.When NaOH solution is added continually for maintaining the pH value at about 7.0 in the fermentation tank,the hydrogen production per medium can reach 2.473 L,which is superior to that in the sample by 5%,with a glucose decomposition rate of 95.2% and a hydrogen mass fraction of 68.73%.The use of organic waste as fermentation substrate has broad economic benefits and environmental benefits.The optimal gas production ability is achieved with a molasses concentration of 35g/L,and the maximum gas volume in the fermentation tank will be up to 22 L.