| 大鸨的随机扩增多态DNA分析与种内亲缘关系研究 |
| |
| 引用本文： | 张方,吴孝兵.大鸨的随机扩增多态DNA分析与种内亲缘关系研究[J].安徽师范大学学报(自然科学版),2005,28(3):316-319. |
| |
| 作者姓名： | 张方 吴孝兵 |
| |
| 作者单位： | 安徽师范大学,生命科学学院,安徽,芜湖,241000;安徽师范大学,生命科学学院,安徽,芜湖,241000 |
| |
| 基金项目： | 国家自然科学基金
,
安徽省优秀青年科研项目
,
安徽省重点实验室基金
,
安徽省学术与技术带头人基金
,
安徽省教育厅自然科学基金 |
| |
| 摘 要： | 利用随机扩增多态DNA技术对大鸨(Otis tarda)6个个体进行了随机扩增多态DNA分析,共筛选出有效随机引物14条,利用所得的随机引物对每只大鸨的基因组DNA进行扩增,共得到327个扩增片段,其中共有片段150个,根据聚类分析所得到的树状图确定了6只大鸨的亲缘关系.
|
| 关 键 词： | 大鸨 亲缘关系 随机扩增多态DNA(RAPD) |
| 文章编号： | 1001-2443（2005）03-0316-04 |
| 收稿时间： | 2004-09-15 |
| 修稿时间： | 2004年9月15日 |
RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS AND THE INTRASPECIFIC RELATIONSHIP OF GREAT BUSTARD(Otis tarda) |
| |
| ZHANG Fang,WU Xiao-bing.RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS AND THE INTRASPECIFIC RELATIONSHIP OF GREAT BUSTARD(Otis tarda)[J].Journal of Anhui Normal University(Natural Science Edition),2005,28(3):316-319. |
| |
| Authors: | ZHANG Fang WU Xiao-bing |
| |
| Institution: | College of Life Science, College of Anhui Normal Universiw, Wuhu 241000, China |
| |
| Abstract: | Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships of 6 Otis tarda. 14 arbitrary primers were screened out and used to amplify genomic DNA of each Bustard.14 primers had clear and distinct RAPD patterns in each amplification. 327 amplified fragments were generated by RAPD and 150 fragments were shared by the 6 Great Bustards. Hierarchical cluster analysis of the RAPD data was used to calculate the similarity coefficient matrix and dendrogram was constructed. The dendrogram indicates the parent relationships of the 6 Great Bustards. |
| |
| Keywords: | otis tarda relationship randomly amplified polymorphic DNA (RAPD) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
