| 携人源TPA基因的慢病毒表达质粒的构建与鉴定 |
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| 引用本文： | 张昱,王燕,杨帆,蔡亚非,王根林.携人源TPA基因的慢病毒表达质粒的构建与鉴定[J].安徽师范大学学报(自然科学版),2010,33(3):267-271. |
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| 作者姓名： | 张昱 王燕 杨帆 蔡亚非 王根林 |
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| 作者单位： | 安徽师范大学,生命科学学院,安徽,芜湖,241000;南京农业大学,动物科技学院,江苏,南京,210095 |
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| 基金项目： | “十一五”国家科技支撑计划项目(No2006BAD04A01,2006BAD04A12); 芜湖市科技计划重点项目(2008620); 安徽省自然科学基金项目(050410201) |
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| 摘 要： | 利用PCR扩增人源TPA基因,再用酶切-连接的方法将目的片段亚克隆入慢病毒表达质粒pL-PGK-eGFP中,最后用测序、酶切和在293细胞中瞬时表达的方法进行了鉴定.结果显示：含人源TPA基因的pL-PGK-TPA-eGFP慢病毒表达载体构建成功,为进一步利用转基因技术更加安全高效地生产TPA,治疗血栓类疾病打下基础.
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| 关 键 词： | 组织型纤溶酶原激活剂 慢病毒载体 基因表达 |
Construction and Identification of the Lentiviral Expression Vector With Human TPA Gene |
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| ZHANG Yu,WANG Yan,YANG Fan,CAI Ya-fei,WANG Gen-lin.Construction and Identification of the Lentiviral Expression Vector With Human TPA Gene[J].Journal of Anhui Normal University(Natural Science Edition),2010,33(3):267-271. |
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| Authors: | ZHANG Yu WANG Yan YANG Fan CAI Ya-fei WANG Gen-lin |
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| Institution: | ZHANG Yu1,WANG Yan1,YANG Fan1,CAI Ya-fei1*,WANG Genlin2*(1.College of Life Sciences,Anhui Normal University,Wuhu 241000,China,2.Animal Science , Technology,College of Nanjing Agriculture University,Nanjing 210095,China) |
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| Abstract: | The gene TPA was amplified by PCR,then the fragment was subcloned into the lentiviral vector pL-PGK-eGFP,and was confirmed by EheI digestion,sequencing and instantaneous expressing.The results of research showed that a lentiviral vector pL-PGK-TPA-eGFP containing human TPA gene was constructed.It will provide foundation for the production of TPA by transgene technology to treat thrombus. |
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| Keywords: | TPA lentiviral vector gene expression |
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