三尖杉酯碱诱导HeLa细胞凋亡的研究

报道了三尖杉酯碱 (harringtonine ,HT)可以诱导HeLa细胞凋亡 .采用Heochst33342荧光染色、琼脂糖凝胶电泳及流式细胞光度术 (FCM)的方法 ,研究了HT对HeLa细胞凋亡的影响 .利用细胞同步化技术和斑点杂交法研究发现 ,HT影响了HeLa细胞c myc和bcl 2基因的表达并且与细胞周期密切相关 .初步探讨其凋亡诱导的机制 ,认为HT通过在G1和G2 期下调细胞凋亡抑制基因bcl 2的诱导凋亡 ,以及下调c myc癌基因阻滞细胞增殖 ,并延迟凋亡发生 .这些结果对于了解HT的药物作用机制和提高临床化疗疗效具有重要意义 .

INDUCTION OF APOPTOSIS BY HARRINGTONINE IN HELA CELLS

The anticancer drug harringtonine(HT) which can induce apopt os is is investigated in HeLa cells by Heochst33342 nucleic staining, agarose gel e lectrophoresis of DNA and flow cytometry. Typical apoptotic cell death is observ ed from 2 to 24 h and it increases in both dose-and time-dependent fashion. The mechanism of HT inducing HeLa apoptosis is studied with synchonized cells a nd measuring gene expression. The results demonstrate that the expression of bcl -2 decreases in G 1 and G 2 and keeps relatively stable in S phase, t his agree with FCM results that HT induces HeLa cell apoptosis and S phase cells increasing. In addition, HT induces down regulation of c-myc to inhibit cell p roliferation and delays the progression of apoptosis. These results are importan t to understand the mechanism of action of HT and to improve the effect of HT in cancer chemotherapy.