| 绿色荧光蛋白基因mgfp4在水稻愈伤组织中的瞬时表达 |
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| 引用本文： | 王泽宙,邱全胜.绿色荧光蛋白基因mgfp4在水稻愈伤组织中的瞬时表达[J].北京师范大学学报(自然科学版),2000,36(3):385-389. |
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| 作者姓名： | 王泽宙 邱全胜 |
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| 作者单位： | 北京师范大学生命科学学院,100875,北京 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金，北京师范大学校科研和教改项目 |
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| 摘 要： | 采用基因枪方法将适用于高等植物的绿色荧光蛋白基因ｍｇｆｐ４转化到水稻愈伤组织之中，获得高效瞬时表达，在荧光显微镜和激光共聚焦显微镜下观察到强烈绿色荧光。绿色荧光蛋白ｍＧＦＰ４生色团形成迅速，转化４ｈ后就能观察到绿色荧光，并且持续时间较长，２ｄ后才基本消退。
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| 关 键 词： | 绿色荧光蛋白 绿色荧光蛋白基因 水稻愈伤组织 基因枪 |
THE TRANSIENT EXPRESSION OF MGFP4 IN RICE CALLI |
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| Wang Zezhou,Qiu Quansheng.THE TRANSIENT EXPRESSION OF MGFP4 IN RICE CALLI[J].Journal of Beijing Normal University(Natural Science),2000,36(3):385-389. |
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| Authors: | Wang Zezhou Qiu Quansheng |
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| Abstract: | High-level expression of mGFP4, a GFP variant suitable for higher plants, is obtained through bombardment transformation into rice calli. Bright green fluorescence is visualized by fluorescence and confocal microscopy. The formation of mGFP4 chromophore is swift and green fluorescence is visualized within 4 h following bombardment. Also, the fluorescence emission lasts for about 2 d. The transformation and expression of mGFP4 are demonstrated by Sou thern blotting and Kanamycin selection experiments. Taken together, rice calli are suitable materials for the transformation and expression of mGFP4, and the success of mGFP4 transformation into rice calli will be valuable to our future research in fusing proteins to GFP and their function analysis in rice cells. |
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| Keywords: | green fluorescent protein mgfp4 rice calli microprojectile bombardment |
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