| 红豆杉愈伤组织生长与PPO,POD比活性和多酚质量分数变化的研究 |
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| 引用本文： | 张月玲,肖尊安,熊红.红豆杉愈伤组织生长与PPO,POD比活性和多酚质量分数变化的研究[J].北京师范大学学报(自然科学版),2002,38(6):800-804. |
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| 作者姓名： | 张月玲 肖尊安 熊红 |
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| 作者单位： | 1. 北京师范大学生命科学学院,100875,北京;2. 西昌农业高等专科学校园艺系,615013,四川,西昌 |
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| 基金项目： | 教育部留学回国人员科研启动基金;; |
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| 摘 要： | 以墨西哥红豆杉(Taxus globosa)和短叶红豆杉(Taxus brerifolia)愈伤组织为材料,在愈伤组织生长的不同时期取样分析生物产量、多酚质量分数、多酚氧化酶(PPO)和过氧化物酶(POD)酶活性.发现墨西哥红豆杉愈伤组织比短叶红豆杉愈伤组织生长快的原因是:多酚质量分数低,POD活性高和PPO活性低.研究表明,通过提高细胞POD活性和降低PPO活性有助于提高红豆杉愈伤组织的生物产量.
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| 关 键 词： | 墨西哥红豆杉 短叶红豆杉 愈伤组织生长 多酚氧化酶 过氧化物酶 |
| 修稿时间： | 2002年3月19日 |
A STUDY ON THE CALLUS GROWTH OF TAXUS AND THE CHANGES IN POLYPHENOL LEVEL AND THE ACTIVITY OF POLYPHENOL OXIDASE AND PEROXIDASE |
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| Zhang Yueling,Xiao Zun'an,Xiong Hong.A STUDY ON THE CALLUS GROWTH OF TAXUS AND THE CHANGES IN POLYPHENOL LEVEL AND THE ACTIVITY OF POLYPHENOL OXIDASE AND PEROXIDASE[J].Journal of Beijing Normal University(Natural Science),2002,38(6):800-804. |
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| Authors: | Zhang Yueling Xiao Zun'an Xiong Hong |
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| Institution: | Zhang Yueling 1) Xiao Zun'an 1) Xiong Hong 2) |
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| Abstract: | Shoot calli of Taxus globosa and Taxus brevifolia are used as materials. Callus fresh weight, polyphenol content, activity of polyphenol oxidase (PPO) and peroxidase (POD) are determined during the period of 7 week cultures. The results show that in comparison with T. Brevifolia, the callus of T. globosa grows more quickly because of its lower level of polyphenol, higher activity of POD and lower activity of PPO. The increase of cell biomass and taxol production by the regulation of the activity of POD or PPO is discussed. |
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| Keywords: | Taxus globosa Taxus brevifolia callus growth polyphenol oxidase peroxidase |
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