大豆黄酮对衰老小鼠脑组织氧化损伤的调节

研究大豆黄酮对D–半乳糖致衰老小鼠脑组织氧化损伤程度的调节.连续背部注射D–半乳糖6周,造衰老模型,然后灌胃大豆黄酮5周(实验组Ⅰ、Ⅱ的剂量分别为5、10,mg/(kg.d)),监测小鼠Y–迷宫记忆、活性氧、丙二醛、脂褐质、8–羟基脱氧鸟嘌呤和不同脑区突触素含量变化,探讨大豆黄酮对衰老小鼠脑组织氧化损伤程度的影响.结果表明,灌胃衰老小鼠大豆黄酮后,Y–迷宫学习记忆能力和大脑皮质突触素含量显著提高,同时脑组织中活性氧、丙二醛、8–羟基脱氧鸟嘌呤和脂褐质含量明显下降.与衰老对照组相比,大脑皮质和海马中的活性氧和8–羟基脱氧鸟嘌呤、脑组织中丙二醛和脂褐质含量降低程度与灌胃剂量具有正相关性.表明大豆黄酮能够提高衰老小鼠学习能力,降低衰老小鼠脑组织氧化损伤程度,具有显著的抗衰老作用.

Regulation of Daidzein on Oxidative Damage Levels in the Brain of Aged Mice

To observe the effects of daidzein on D-galactose induced senescence mice,D-galactose was for 6 weeks injected into the dorsal of mice as aged models.The aged mice were fed with different doses(5,10,mg/(kg.d))of daidzein for 5,weeks.Y-maze was tested and the levels of synatophysin,reactive oxygen species,malondialdehyde,lipofuscin,and 8hydroxy deoxyguanosine in brain were examined.The results showed that daidzein could increase Y-maze memory ability and the synatophysin level in the cortex,decrease reactive oxygen species and 8-hydroxy deoxyguanosine levels in the cortex and hippocampus of the aged mice,and also decrease the malondialdehyde and lipofuscin levels in brain tissues.Daidzein could increase memory ablility and decrease oxidative damage of the mice’s brain.Daidzein could antisenesce in some degree.