| 改性聚苯乙烯微球对蛋白质的吸附与解吸特性 |
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| 引用本文： | 程绍玲,倪丽琴,杨迎花.改性聚苯乙烯微球对蛋白质的吸附与解吸特性[J].天津科技大学学报,2010,25(1):28-31. |
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| 作者姓名： | 程绍玲 倪丽琴 杨迎花 |
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| 作者单位： | 天津科技大学理学院,天津,300457 |
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| 基金项目： | 天津科技大学引进人才科研启动基金资助项目 |
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| 摘 要： | 通过蒸馏–沉淀法制备单分散的聚苯乙烯(PS)微球,并用两亲性聚合物聚乙二醇(PEG)对PS进行修饰.以牛血清蛋白(BSA)为模型,研究PS-PEG微球对BSA的吸附与解吸特性.结果表明,聚合物微球对BSA的吸附受pH、微球上PEG含量以及NaCl溶液质量浓度的影响,作用力为疏水吸附.在无盐水体系下解吸,解吸率最高为96.2%,表明微球在蛋白质分离应用中可重复使用.
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| 关 键 词： | 聚合物微球 吸附 疏水作用 蛋白质 |
Adsorption and Desorption of Proteins on Modified Polystyrene Microspheres |
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| CHENG Shao-ling,NI Li-qin,YANG Ying-hua.Adsorption and Desorption of Proteins on Modified Polystyrene Microspheres[J].Journal of Tianjin University of Science & Technology,2010,25(1):28-31. |
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| Authors: | CHENG Shao-ling NI Li-qin YANG Ying-hua |
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| Institution: | CHENG Shao-ling,NI Li-qin,YANG Ying-hua(College of Science,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | Monodisperse polystyrene microspheres(PS)were prepared by distillation-precipitation polymerization and modified by amphipathic polymers polyethylene glycol(PEG).Bovine serum albumin(BSA)was used as model proteins to investigate the adsorption and desorption properties on PS-PEG microspheres.As a result,BSA adsorption on polymer microspheres was driven by hydrophobic interaction and effected by the value of pH,the capacities of PEG on the microspheres and inorganic salt concentration.The maximum desorption ... |
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| Keywords: | polymer microsphere adsorption hydrophobic interaction protein |
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