| 一株分离自潮间带污泥细菌Clostridium sp. T7产氢特性分析 |
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| 引用本文： | 刘洪艳,王文磊.一株分离自潮间带污泥细菌Clostridium sp. T7产氢特性分析[J].天津科技大学学报,2011,26(6):12-15. |
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| 作者姓名： | 刘洪艳 王文磊 |
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| 作者单位： | 天津市海洋资源与化学重点实验室,天津科技大学海洋科学与工程学院,天津300457 |
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| 基金项目： | 天津市海洋资源与化学重点实验室开放基金，天津科技大学实验室开放基金 |
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| 摘 要： | 产氢菌株Clostridium sp. T7分离自天津海水浴场潮间带的污泥.研究起始pH、碳源、氮源、NaCl质量分数对菌株T7产氢性质的影响.结果表明:菌株T7最适产氢的起始pH是6.0,能够利用蔗糖、葡萄糖和果糖等碳源发酵产氢.菌株T7能够利用牛肉膏和酵母粉为单一氮源产氢,不能利用蛋白胨为氮源进行产氢.NaCl质量分数能影响菌株T7的产氢量,海水培养条件(NaCl质量分数为3%)下,最高产氢量是每摩尔葡萄糖(1.48,±0.05)mol,相比之下,淡水培养条件下其产氢量提高20%.NaCl质量分数在0.4%～7%时,菌株T7都能够产氢,这表明菌株T7有望应用于淡水或高盐有机废水产氢领域.
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| 关 键 词： | 潮间带污泥 产氢菌株 产氢量 |
Characterization of Hydrogen Production of a Strain Clostridium sp.T7 Isolated from Intertidal Sludge |
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| LIU Hong-yan,WANG Wen-lei.Characterization of Hydrogen Production of a Strain Clostridium sp.T7 Isolated from Intertidal Sludge[J].Journal of Tianjin University of Science & Technology,2011,26(6):12-15. |
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| Authors: | LIU Hong-yan WANG Wen-lei |
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| Institution: | LIU Hong-yan,WANG Wen-lei(Tianjin Key Laboratory of Marine Resources and Chemistry,College of Marine Science and Engineering,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | A hydrogen-producing bacterium,Clostridium sp.T7,was isolated form the sludge collected from intertidal zone of a bathing beach in Tianjin.The effects of initial pH value,carbon source,nitrogen source and NaCl concentration on hydrogen production of strain T7 were determined in this study.The results showed that strain T7 produced hydrogen production at maximal level when growing at initial pH of 6.0.The strain was able to produce hydrogen using many carbon sources,such as glucose,sucrose and fructose.The s... |
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| Keywords: | intertidal sludge hydrogen-producing strain hydrogen production |
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