| 发酵液中鸟苷和肌苷的分离测定 |
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| 引用本文： | 刘辉,王颖,张蓓,刘淑云,陈宁,张克旭.发酵液中鸟苷和肌苷的分离测定[J].天津科技大学学报,2004,19(4):18-21. |
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| 作者姓名： | 刘辉 王颖 张蓓 刘淑云 陈宁 张克旭 |
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| 作者单位： | 天津科技大学生物工程学院,天津,300222 |
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| 基金项目： | 天津市高等学校科技发展基金项目(20031205) |
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| 摘 要： | 初步选择了HD-8和HZ014两种树脂进行鸟苷和肌苷分离测定实验，通过模糊聚类对实验结果分析得到分离测定操作条件的较优区域：再选择适当的条件进行静态吸附实验，从3种树脂HD-8、HZ014和732中最终确定采用树脂HD-8．通过模糊聚类产生的较优区域中的不同务件下实验结果的比较，最后确定最佳分离条件为：采用树脂HD-8，树脂床高度为4cm．流速为1mL／min，前120mL采用0．05mol／L盐酸洗脱收集肌苷，120mL后至240mL采用0．74moL／L pH5．0醋酸钠缓冲液洗脱收集鸟苷。
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| 关 键 词： | 离子交换法 肌苷 鸟苷 分离 模糊聚类分析 |
| 文章编号： | 1672-6510(2004)04-0018-04 |
| 修稿时间： | 2004年5月10日 |
Studies on the Seperation of Guanosine and Inosine in Fermentation Broth by Ion Exchange |
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| LIU Hui,WANG Ying,ZHANG Bei,LIU Shu-yun,CHEN Ning,ZHANG Ke-xu.Studies on the Seperation of Guanosine and Inosine in Fermentation Broth by Ion Exchange[J].Journal of Tianjin University of Science & Technology,2004,19(4):18-21. |
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| Authors: | LIU Hui WANG Ying ZHANG Bei LIU Shu-yun CHEN Ning ZHANG Ke-xu |
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| Abstract: | The better region of isolation and determination of inosine and guanosine in the fermentation broth was achieved by applying the fuzzy clustering analysis to the experiment. The new type of resin HD-8 was finally chosen. Because its effect was better than resin HZ014 and resin 732 in the captive test. Then research was further done by chosing several conditions according to the better region. Finally the optical condition was determined:the height of resin was 4 centimeter, the rate of flow was 1 mL/min,the inosine was obtained by collecting the first 120 mL eluant of 0.05 mol/L HCI and then flush solution was changed by pH5.0 NaAc buffer. guanosine was obtained froml20 mL to 240 mL. |
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| Keywords: | inosine guanosine ion exchange separation fuzzy clustering analysis |
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