龙虎泡油田微生物驱油评价实验

为了在大庆龙虎泡油田应用微生物采油技术,进行室内评价实验。从龙虎泡油田地层水中筛选到一株内源菌DH8,经鉴定为Geobacillusstearothermophilus,另外从菌种库中挑选一株适合该油藏条件生长的外源菌SL21,经鉴定为Bacillus licheniformis。室内对DH8和SL21的乳化性能、降解能力和驱油效率进行了评价。研究发现,菌株DH8和SL21发酵液的比毛管数分别为0.0239 s/m和0.0332 s/m;菌株DH8和SL21分别使原油黏度降低19.02%和26.08%;气相色谱分析表明菌株DH8主要降解C_(13)~C_(27)内正构烷烃,而菌株SL21主要降解C_(19)~C_(37)内正构烷烃,经DH8和SL21降解后,C_(13)之前烷烃含量占比分别增加了5.33%和11.00%;物模实验表明,菌株SL21和菌株DH8的驱油效率分别为7.11%和5.50%。综合分析得出外源菌SL21的各项驱油性能指标均优于内源菌DH8。

Experimental evaluation of the microbial oil displacement in Longhupao oilfield

In order to apply the technology of the microbial enhanced oil recovery in the Longhupao oilfield of Daqing, a series of experiments are conducted in our laboratory. Two strains of microbes are considered in the experiment. The endogenous microbe DH8 is screened from the Longhupao oilfield formation water, identified as the Geobacillusstearothermophilus; the inoculating microbe SL21 is selected from the strain library, identified as the Bacillus licheniformis. Their emulsification property, degradation ability and oil displacement efficiency are studied. It is shown that the specific capillary numbers of the DH8 and the SL21 are 0.0239 s/m and 0.0332 s/m, respectively; the viscosity reductions of the oil are 19.02% and 26.08% after the applications of the DH8 and the SL21. The linear Alkanes in the C₁₃~C₂₇ are mainly degraded by the DH8, and the linear Alkanes in the C₁₉~C₃₇ are mainly degraded by the SL21, but the short-chain n-alkanes before the C₁₃ are both increased by 5.33% and 11.00% after the applications of the fDH8 and SL21. The physical flooding simulation experiments show that the oil displacement efficiency of the SL21 and the DH8 are 7.11% and 5.5%, respectively. Therefore, the oil displacment performance of the SL21 is better than that of the DH8.