高尿酸血症大鼠模型血清代谢组学研究

 通过检测高尿酸血症大鼠血清代谢谱的变化, 探讨代谢组学在高尿酸血症研究中的应用。将20 只清洁级健康SD(Sprague-Dawley)大鼠随机分为高尿酸血症模型组(10 只)和空白对照组(10 只), 每日采用酵母膏(YEP)21 g·kg^-1·d^-1给予喂养, 氧氢酸钾(OA)以200 mg-1·kg^-1·d^-1腹腔注射1 次, 建立高尿酸血症大鼠模型。第28 日收集空白对照组和模型组大鼠血清, 进行¹H-NMR 谱测定和主成分分析。与空白对照组大鼠相比, 模型组大鼠血清中糖蛋白、丙酮酸、丙氨酸、异亮氨酸、亮氨酸显著增加, 而甲酸浓度及极低密度脂蛋白、不饱和脂肪酸、丙酮显著降低。由此得出, 高尿酸血症大鼠血清代谢组学发生明显变化, 血浆代谢组学在一定程度上反映高尿酸血症大鼠的病理生理变化。

Serum metabonomics research of hyperuricemia rat model

The serum metabonomic variation in hyperuricemia rats was investigated in order to explore the application of metabonomics in research of hyperuricemia. The animals were divided into two groups at random, i.e., the normal group (n=10) and the model group (n=10). The rat model was treated with yeast extract powder(YEP)as 21 g·kg^-1·d^-1 and OA paired 20 g/L mother liquor with sterile distilled water. The two group's serum was collected and its ¹H nuclear mannetic resonance (¹H-NMR) spectra was acquired. Compared with the normal control group, the model group′s glycoprotein, pyruvic acid, alanine, isoleucine, leucine were all increased, however, formaldehyde, VLDL, unsaturated fatty acid, and acetone were reduced. Significant changes in serum metabonomics found in the hyperuricemia rat models might reflect to a certain extent the pathological changes of hyperuricemia rat models.