曲霉属产毒真菌DNA的制备及其毒素相关基因aflR的PCR 检测

 研究了用改良的CTAB 法提取曲霉属产毒真菌DNA 的方法，并与试剂盒提取法进行比较。经过改良的CTAB 法能够成功提取出曲霉属产毒真菌的DNA，且浓度和纯度均可达到PCR 扩增的标准。根据合成黄曲霉毒素的aflR 基因设计两对引物，运用PCR 方法进行扩增鉴定，结果表明黄曲霉、寄生曲霉和溜曲霉基因中可检出aflR 基因，烟曲霉和杂色曲霉中并未检出，达到对黄曲霉毒素产生菌进行鉴定的目的。这种方法可为产毒真菌的快速检测提供参考。

DNA Preparation of Toxigenic Aspergillus and PCR Detection of aflR Toxin Gene

This paper discusses the modified CTAB method for DNA extraction of toxigenic Aspergillus and compares it with the kit extraction method. The modified CTAB method successfully extracted DNA of toxigenic Aspergillus. The concentration and purity reached the standard of PCR amplification. Two pairs of primers were designed based on the aflR gene for aflatoxin biosynthesis regulation, and the toxigenic Aspergillus aflR gene was identified by using PCR amplification. The results show that the aflR gene is contained in the genome of Aspergillus flavus, Aspergillus parasitic and Aspergillus tamarii, Aspergillus fumigates and Aspergillus versicolor are not detected, realizing the purpose for identifying aflatoxin producing strains. This method provides a reference for rapid detection of toxigenic fungi.