| 大鼠背根神经节神经元的分离方法及电生理特征探讨 |
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| 引用本文： | 王腾,黄从新,王高华.大鼠背根神经节神经元的分离方法及电生理特征探讨[J].科技导报(北京),2009,27(16):32-35. |
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| 作者姓名： | 王腾 黄从新 王高华 |
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| 作者单位： | 王腾,黄从新(武汉大学人民医院心内科,武汉,430060;武汉大学心血管病研究所,武汉,430060);王高华(武汉大学人民医院精神卫生中心,武汉,430060) |
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| 摘 要： | 在获取大鼠背根神经节神经元后,全细胞膜片钳技术记录动作电位和钠电流,探讨大鼠背根神经节细胞的分离方法和细胞形态以及电生理特征.结果显示,本实验能得到完整圆形或椭圆长条形的大鼠背根神经节体.正常的单个背根神经节神经元呈圆形或椭圆形,大小不等,胞膜清晰,折光性好,隐约可见细胞核.在背根神经节细胞上记录的动作电位呈正立锐角三角形,静息电位小,动作电位时程短.背根神经节神经元的钠通道最大电流密度为(-62.04±4.45)pA/pF,几乎能被河豚毒素(TTX)完全抑制.本实验分离方法简单易行,背根神经节神经元容易获得和辨认,电生理特征明确,适用于神经系统疾病的电生理特征研究和治疗药物观察.
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| 关 键 词： | 膜片钳技术 背根神经节神经元 大鼠 电生理特征 |
Isolation Method of Dorsal Root Ganglion Neurons of Rats and Their Electrophysiological Characteristics |
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| WANG Teng,HUANG Congxin,WANG Gaohua.Isolation Method of Dorsal Root Ganglion Neurons of Rats and Their Electrophysiological Characteristics[J].Science & Technology Review,2009,27(16):32-35. |
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| Authors: | WANG Teng HUANG Congxin WANG Gaohua |
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| Institution: | WANG Teng1,2,HUANG Congxin1,WANG Gaohua3 1.Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060,China 2.Cardiovascular Research Institute,Wuhan University,China 3.Mental Health Centre,China |
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| Abstract: | To investigate the isolation method of Dorsal Root Ganglion(DRG) neurons in rats and their electrophysiological characteristics, DRGs were obtained by double enzymatic dissociation.The patch clamp technique was used to record Action Potentials(AP) and sodium currents INa.The results show that a body of round and long DRG, and DRG neurons with middle measures and clear membranes and nucleus was obtained with good refraction properties.APs recorded in DRG neurons consist of five phases and inshape of upward a... |
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| Keywords: | patch-clamp technique dorsal root ganglion neuron rat electrophysiological characteristics |
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