食管鳞状细胞癌中VEGF和nm23表达和临床意义

探讨VEGF和nm23基因产物表达与食管鳞状细胞癌(ESCC)临床病理指标及预后的关系,应用免疫组织化学Elivision法对食管癌组织和癌旁正常食管粘膜组织中VEGF蛋白和nm23蛋白进行检测。结果表明,90例食管癌组织中VEGF蛋白56例高表达,19例低表达,15例不表达,阳性率为83.33%,在28例正常黏膜组织中6例低表达,阳性率为21.42%,两者比较具有统计学意义(P0.05);90例食管癌组织中nm23蛋白24例高表达,23例低表达,43例不表达,阳性率为52.22%,在28例正常食管黏膜组织中23例表达,5例不表达,阳性率为82.14%,两者比较具有统计学意义(P0.05)。无食管癌旁淋巴结转移组的nm23阳性表达率64%(32/50)较有转移组的表达率37.5%(15/40)高,有显著性差异(P0.05)。VEGF和nm23的表达与分化程度、浸润深度无关(P0.05)。由此表明,VEGF阳性伴nm23阴性表达的患者发生淋巴结转移的可能性大,VEGF和nm23在食管癌中的表达与食管癌的发生、发展、浸润、转移和预后密切相关,可作为临床预测浸润转移和估计预后的重要参考指标。

Expressions of Vascular Endothelial Growth Factor and Non-Metastasis 23 in Eesophageal Squamous Cell Carcinoma and Their Clinical Significance

This paper investigates the expressions of Vascular Endothelial Growth Factor (VEGF) and non-metastasis 23 in esophageal squamous cell carcinoma and their clinical significance. The expressions of VEGF and nm23 proteins were detected by Elivision immunohistochemical method. The positive rate of VEGF protein in 90 cases of ESCC was 83.33%, with 56 cases of high expression, 19 cases of low expression and 15 cases of no expression. The positive rate of VEGF protein in 28 cases of normal esophageal epithelium was 21.42% (6/28) (P<0.05). The positive rate of nm23 protein in 90 cases of ESCC was 52.22 %, with 24 cases of high expression, 23 cases of low expression, and 43 cases of no expression. The positive rate of nm23 protein in 28 cases of normal esophageal epithelium was 82.14% (23/28) (P<0.05). The positive rate of nm23 protein in ESCC was 64% (32/50) with lymph node metastasis. The rate of nm23 protein positive expression was 37.5%(15/40) in the carcinomas without lymph node metastasis(P<0.05). Their expressions were not correlative with differentiation degree and depth of invasion(P>0.05). The results indicate that the positive expression of VEGF protein with nm23 negative expression means a higher possibility of LNM. The expressions of VEGF and nm23 proteins are correlated with the depth of invasion, lymph node metastasis and clinical staging in ESCC(P＜0.05), but not with differentiation. They both have a close correlation with carcinogenesis, development, infiltration, metastasis and prognosis and might have predictive value for prognosis of human ESCC.