甘遂半夏汤中甘遂与甘草不同比例配伍对癌性腹水模型大鼠肝功能的影响

 通过甘遂与甘草不同比例配伍的甘遂半夏汤对腹水模型大鼠肝功能相关指标的检测,筛选甘遂与甘草反药组合对肝毒性影响的配比宜忌条件。将甘遂与甘草按照2因素7水平的均匀设计实验原则设置不同配伍比例,观察甘遂与甘草不同配比的甘遂半夏汤对癌性腹水模型大鼠血清转氨酶、总蛋白(TB)、白蛋白(ALB)、球蛋白(GLB)及肝组织病理形态的影响,并利用中药组方优化软件对生物效应指标进行甘遂与甘草配比的优化分析。研究表明,配比1组(10.40﹕1.17)和配比6组(20.80:0.78)较模型组显著降低谷草转氨酶(AST)水平(P < 0.05);配比4组(3.47:0.39)较模型组显著增加谷丙转氨酶(ALT)、ALB水平(P < 0.05);配比5组(0.21:1.56)、配比7组(17.34:1.94)及呋塞米组较模型组显著降低ALT水平;但各给药组AST/ALT与模型组无显著性差异(P > 0.05);病理形态变化显示,配比2组(6.94:2.33)、配比5组(0.21:1.56)、配比7组(17.34:1.94)及呋塞米组较模型组病变程度重。结果显示,甘遂用量在1.5g以上的配比组,肝细胞组织结构损伤较其他给药组严重,提示配比组的肝损伤作用可能与甘遂用量过大有关。

Effect of Gansuibanxia Decoction with Different Proportions of Gansui to Gancao on Hepatic Functions in Malignant Ascites Rats

The effect of gansuibanxia decoction with different proportion of Gansui to Gancao on malignant ascites rats is investigated. Seven matched groups are set up according to the principle of uniform design (two factors and seven levels) for comparing normal group with model and positive groups. At the end of experiment, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), and globulin (GLB) are determined by the automatic analyzer. The data statistic analysis is processed by using ANOVA (analysis of variance) in combination with optimized formula software. The experimental data show that matched group one and group six are able to decrease AST compared with model group; matched group four could increase ALT and ALB;matched group five, group seven and group with Frusemide sodium could decrease ALT; but there is no significant difference among every groups;matched group two, group five, group seven, and group with Frusemide sodium have a significant impact on the tissue of liver compared with model group. Through the results analysis of animal experiments, following conclusions are obtained that the matched groups including the dose of gansui greater than 1.5g could have a certain degree liver injury. It is suggested that gansui might play a role in the liver injury.