西芹根际区物浸提液处理后黄瓜叶片内酚类物质、氮代谢物质含量的变化

 以黄瓜品种津春4号为材料,在黄瓜第1片真叶长至横宽5cm时,使用西芹根际区物的蒸馏水、丙酮和乙醇浸提液对黄瓜植株进行化感处理(灌根),测定处理后黄瓜叶片内几种酚类物质和氮代谢物质含量的变化,包括木质素、单宁、阿魏酸、绿原酸和氨基酸、可溶性蛋白质、氨、硝酸盐,以研究西芹根际区物浸提液处理后黄瓜植株对黄瓜枯萎病菌的化感抑制作用机制.结果表明,经西芹根际区物浸提液处理(灌根)黄瓜植株后,黄瓜叶片内酚类物质含量均升高;黄瓜叶片内氮代谢物质含量亦均有变化,含量升高的物质有精氨酸、脯氨酸、可溶性蛋白质和氨,而甘氨酸、缬氨酸、蛋氨酸、异亮氨酸、苯丙氨酸和硝酸盐含量则低于对照;对于不同处理种类,丙酮浸提液处理的物质含量变化最显著,乙醇浸提液处理次之,蒸馏水浸提液处理变化最低.

Changes of Phenol and Nitrogen Metabolites Contents in Cucumber Leaves Treated with the Extracts of Celery Root Soil

Previous studies show that the celery extracts have the allelopathic effect on the Fusarium oxysporium f. sp. Cucumerinum with an inhibitory rate of 12.89%-84.83% and different fresh celery root and root soil extracts have different prohibitive allelopathic strengths against the cucumber wilt. With a steep decline in morbidity, however, the prohibitive allelopathic strengths are within 5.45%-46.95%. An interesting issue is the mechanism of the celery extract allelopathy, including the protective enzyme systems, the soluble substances and the active oxygen metabolism of the cucumber leaf, which were studied by researchers However, little attention was paid to the phenol and nitrogen metabolites in the cucumber leaf To study the mechanisms of the celery root soil extracts allelopathy on the Fusarium oxysporium f. sp. Cucumerinum, the changes in the phenol metabolites and the nitrogen metabolites were analyzed in "Jinchun 4"cucumber leaves treated with the extracts once its first leaf was 5cm wide, including the lignin, the tannin, the chlorogenic acid, the ferulic acid, the amino acid, the soluble protein, the ammonia, the nitrate and the nitrite. It is shown that the phenol metabolites in the cucumber leaves treated with the celery root soil extracts are significantly higher those without the treatment, the content of the nitrogen metabolites changes significantly, the contents of the Arg, the Pro, the soluble protein, and the ammonia are higher, but the contents of the Gly, the Val, the Met, the Ile, the Phe and the nitrate are lower. For different treatments, the most significant effects on the phenol metabolites and the nitrogen metabolites are caused by the acetone extracts, followed by the ethanol extracts and the distilled water extracts.