河北省和黑龙江省马铃薯晚疫病菌SSR基因型分析

用SSR分子标记,对致病疫霉群体结构进行分析,完善了中国研究晚疫病菌的分子标记体系。利用两个SSR引物Pi4B和Pi4G对来自河北和黑龙江两个省份的58个马铃薯晚疫病菌株基因组DNA进行SSR基因型鉴定,分析其遗传多样性。河北省的菌株共鉴定出4个SSR基因型:D-03,F-01,G-02和F-06。其中,F-06是首次报道的新的SSR基因型。黑龙江的菌株共鉴定出2个SSR基因型。在两省的菌株中,基因型F-01所占比例都最大,且占绝对优势。不同交配型可存在于同一SSR基因型中,同一SSR基因型的菌株对甲霜灵的敏感性存在差异。聚类结果还显示占被测菌株群体最大比例的F-01基因型与国外的对照菌株的亲缘关系较远。

Genotypic Analysis of Population of Phytophthora infestans in Hebei and Heilongjiang Based on SSR

The population structure of P. infestans is analyzed with SSR molecular mark to fill a gap in the molecular marker system. The SSR genotypes of the genomic DNA of 58 isolates of P. infestans from Hebei and Heilongjiang were identified using the SSR primers of Pi4B and Pi4G in analyzing the polymorphism. Four SSR genotypes of isolates from Hebei were identified,which are D-03,F-01,G-02 and F-06. SSR genotype F-06 was a new genotype,which is first reported in this paper. Two SSR genotypes of isolates from Heilongjiang were identified. F-01 was the dominant SSR genotype of the isolates from the two Provinces. The isolates of the same SSR genotype may belong to different mating types,A1 or A2,and may be on different sensitivity levels with respect to Metalaxyl. The SSR genotype F-01,which dominates the P. infestans population,was found to be far from the reference isolates of foreign countries in phylogenesis by clustering analysis.