| 产漆酶菌株的筛选及产酶条件的优化 |
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| 引用本文： | 王巍杰,张杰.产漆酶菌株的筛选及产酶条件的优化[J].河北理工学院学报,2014(2):81-83. |
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| 作者姓名： | 王巍杰 张杰 |
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| 作者单位： | [1]河北联合大学生命科学学院,河北唐山063009 [2]河北联合大学化学工程学院,河北唐山063000 |
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| 摘 要： | 以愈创木酚培养基为底物，利用平板筛选法从土壤朽木中筛选能够产漆酶的菌株，通过测定漆酶活力进行复筛，筛选出一株产漆酶活力较高的白腐茵菌株。以漆酶活性为参考指标，确定葵花粉发酵产漆酶条件：发酵培养基中硫酸铵0．025％，初始pH值5．5，接种量8％，装液量50mL，愈创木酚0．10％，培养时间7d。
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| 关 键 词： | 漆酶 筛选 白腐茵 酶活测定 |
Screening and Otimization of Frmentation Cnditions of a Lccase-producing Srain |
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| WANG Wei-jie,ZHANG Jie.Screening and Otimization of Frmentation Cnditions of a Lccase-producing Srain[J].Journal of Hebei Institute of Technology,2014(2):81-83. |
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| Authors: | WANG Wei-jie ZHANG Jie |
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| Institution: | 2 ( 1. College of Life Sciences, Hebei United University,Tangshan Hebei 063009, China; 2. School of Chemical Engineering, Hebei United University, Tangshan Hebei 063000, China) |
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| Abstract: | To guaiacol as the substrate, a laccase-producing strain was screened by medium flats and rescreened by measuring the laccase activity from soil and rotten wood samples. A high yield white-rot fungus for laccase production was screened. As a reference index to determining the laccase activity, a better culturing condition was obtained. The results were ammonium sulfate 0. 025%, culture initial pHS. 5, inoculum 8% ,250 mL flask with 50mL medium, guaiacol 0.10% and the culture time 7d. |
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| Keywords: | laccase screening white-rot fungi laccase activity measurement |
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