| 毕赤酵母表达重组植酸酶酶学性质的比较研究 |
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| 引用本文： | 邹由,付玲,江维,周玉玲,马立新.毕赤酵母表达重组植酸酶酶学性质的比较研究[J].湖北大学学报(自然科学版),2011,33(2):187-189,208. |
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| 作者姓名： | 邹由 付玲 江维 周玉玲 马立新 |
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| 作者单位： | 湖北大学生命科学学院,湖北武汉,430062 |
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| 摘 要： | 通过酶学性质比较毕赤酵母GS115表达的E.coli K12植酸酶(appA)及其突变体植酸酶(appA NR)和黑曲霉植酸酶(phyA)的最适pH、温度、热稳定性及对胃蛋白酶和胰蛋白酶的耐受性.结果表明,在95℃,加热10 min,appA NR可残余90％左右的活力,而其他两种只残余20％～50％的活力；appAN...
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| 关 键 词： | 重组植酸酶 毕赤酵母 酶学性质 比较 |
Comparative studies on the enzymology properties of severval phytases expressed by Pichia pastoris |
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| ZOU You,FU Ling,JIANG Wei,ZHOU Yuling,MA Lixin.Comparative studies on the enzymology properties of severval phytases expressed by Pichia pastoris[J].Journal of Hubei University(Natural Science Edition),2011,33(2):187-189,208. |
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| Authors: | ZOU You FU Ling JIANG Wei ZHOU Yuling MA Lixin |
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| Institution: | (School of Life Science,Hubei University,Wuhan 430062,China) |
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| Abstract: | The enzymology properties of three crude recombinate phytase preparations were compared.The encoding genes of the enzyme were E.coli K12 appA wild type,appA mutation appA NR,and Aspergillus niger phyA,and heterogenous expressed in Pichia pastoris.The enzymes were studied for their pH behaviour,temperature optimum,thermostability and resistance to protease inactivation and found to exhibit different properties depending on source of the phytase gene.The results showed that appA NR were more thermostable,pH tolerance,and trypsinase and pepsin resistant than compared to other enzymes.So,appA NR with properties of high temperature stability,broad pH range and high proteolytic stability is favourable for future applications as feed additives. |
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| Keywords: | recombinate phytase Pichia pastoris enzymology properties compare |
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