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柑桔黄龙病病原——类细菌(Bacterium—like organism,BLO)的培养与性质
引用本文:赵鸿燕,周咏芝,葛宗民,刘瑜,丁达明.柑桔黄龙病病原——类细菌(Bacterium—like organism,BLO)的培养与性质[J].湖北大学学报(自然科学版),1990(1).
作者姓名:赵鸿燕  周咏芝  葛宗民  刘瑜  丁达明
作者单位:中国科学院武汉病毒研究所 (赵鸿燕,周咏芝,葛宗民,刘瑜),中国科学院武汉病毒研究所(丁达明)
摘    要:采用体外培养的方法首次从患柑桔黄龙病的柑桔树叶中分离并培养出柑桔黄龙病的病原——类细菌(BLO),测定了BLO最适培养条件及形状、大小和壁膜厚度.将培养出的BLO回接到指示植物长春花,2个月后长春花表现症状,且能重新培养出该BLO。健康柑桔树叶片及长春花均未培养出BLO。

关 键 词:柑桔黄龙病  类细菌  培养

CULTURE AND CHARACTERISTICS OF BACTERIUM-LIKE ORGANISM (BLO) FROM CITRUS YELLOW SHOOT
Zhao Hongyan Zhou Yongzhi Ge Zongmin Liu Yu Ding Darning.CULTURE AND CHARACTERISTICS OF BACTERIUM-LIKE ORGANISM (BLO) FROM CITRUS YELLOW SHOOT[J].Journal of Hubei University(Natural Science Edition),1990(1).
Authors:Zhao Hongyan Zhou Yongzhi Ge Zongmin Liu Yu Ding Darning
Abstract:Using the method of culture in vitro, bacterium-like organism (BLO) ciusing citrus yellow shoot disease can be isolated and cultivated. The optium conditions,the morphology,the sizes and the thick of wall-membrane of cultured BLO can be measured. Cultured BLO inoculated into the leaves of indicated periwinkle, a sensitive host to citrus yellow shoot (BLO). After two months, the infected periwin kle showed typical disease symptoms,and the BLO can be recultured from the diseased leaves of infect ed periwinkle. BLO can not be isolated and cultured from healthy citrus and periwinkle.
Keywords:Citrus Yellow Shoot  Berium-like Organism  Culture in vitro    
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