| 丝石竹花外植体诱导丛生芽的研究 |
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| 引用本文： | 刘幼琪,万永红,陈冬玲,蔡得田.丝石竹花外植体诱导丛生芽的研究[J].湖北大学学报(自然科学版),2001,23(3):269-272. |
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| 作者姓名： | 刘幼琪 万永红 陈冬玲 蔡得田 |
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| 作者单位： | 湖北大学生命科学学院，湖北武汉430062 |
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| 基金项目： | 武汉市科委科技攻关项目 |
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| 摘 要： | 研究了在不同外源激素条件下诱导丝石竹花朵恢复营养生长的过程，结果表明单一生长素仅能诱导花产生愈伤组织，6－BA或6－BA与生长素组合能在完全盛开的诱导花在部产生腑芽并发育成营养枝，在未开放花中能诱导花瓣回复到叶的状态，花瓣已开始萎蔫的花不能诱导逆转。
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| 关 键 词： | 丝石竹 外源激素 丛生芽 生长素 营养生长 愈伤组织 诱导效应 |
| 文章编号： | 1000-2375(2001)03-0269-04 |
| 修稿时间： | 2000年12月28 |
Studies on formation of the multiple-buds from flowers of Gypsophila elegans bieb |
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| Liu Youqi,Wan Yonghong,Chen Dongling,Cai Detian.Studies on formation of the multiple-buds from flowers of Gypsophila elegans bieb[J].Journal of Hubei University(Natural Science Edition),2001,23(3):269-272. |
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| Authors: | Liu Youqi Wan Yonghong Chen Dongling Cai Detian |
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| Abstract: | The effects of some auxins and cytokinins on formation of multiple-buds in Gypsophila elegans Bieb. were investigated. The experiments revealed:only auxins could not induce buds germination in flowers. 6-BA?2~{!+~}3?mg~{!$~}L~{*,*)~}-1~{**~} had good effects on promotion of multiple-buds. Petals were three times bigger than originals under the effects of KT or ZT. The strong multiple-buds were fromatted on MS medium containing NAA 0.2?mg~{!$~}L~{*,*)~}-1~{**~},6-BA?3?mg~{!$~}L~{*,*)~}-1~{**~}. The paraffin-section of explants cultured about 3~{!+~}7 days and stained with hematoxylin demonstrated the buds grew out from base of filaments. |
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| Keywords: | flowers of Gypsophila elegans Bieb hormone multiple-buds |
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