| ITS2序列鉴定青葙子及其混伪品 |
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| 引用本文： | 刘义梅,陈倩,陈科力,陈士林.ITS2序列鉴定青葙子及其混伪品[J].湖北大学学报(自然科学版),2011,33(3):309-312,350. |
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| 作者姓名： | 刘义梅 陈倩 陈科力 陈士林 |
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| 作者单位： | 1. 湖北中医药大学中药资源和中药复方教育部重点实验室,湖北武汉,430065
2. 中国医学科学院北京协和医学院药用植物研究所,北京,100193 |
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| 基金项目： | 国际科技合作项目(2007DFA30990); 卫生行业科研专项(200802043)资助 |
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| 摘 要： | 为探讨基于ITS2序列鉴定青葙子及其混伪品的新方法,对青葙子及其混伪品共4个物种7份样品的ITS2序列进行PCR扩增和测序,同时从GenBank下载青葙子及其常见混伪品共10个物种21个样本的序列信息.分析各物种间ITS2序列二级结构的差异,最后利用ITS2序列构建其系统发育树.结果表明青葙子及其混伪品的ITS2二级结...
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| 关 键 词： | 青葙子 ITS2序列 混伪品 分子鉴定 |
Identification of Celosia argentea and its adulterants by ITS2 sequence |
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| LIU Yimei,CHEN Qian,CHEN Keli,CHEN Shilin.Identification of Celosia argentea and its adulterants by ITS2 sequence[J].Journal of Hubei University(Natural Science Edition),2011,33(3):309-312,350. |
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| Authors: | LIU Yimei CHEN Qian CHEN Keli CHEN Shilin |
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| Abstract: | To discriminate Celosia argentea and its adulterants using ITS2 sequence.DNA samples were extracted from Celosia argentea.ITS2 sequence was amplified by PCR and sequenced bidirectionally.Sequences were assembled by CodonCode Aligner.ITS2 secondary structures were predicted by the web tool established by Schultz etc and NJ tree was constructed by MEGA V 4.1.The results showed that Celosia argentea could be differentiated obviously from its adulterants by their ITS2 secondary structure.Different samples of Ce... |
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| Keywords: | Celosia argentea ITS2 adulterants molecular identification |
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