| 孔雀石绿在核酸表面分子的长距组装及其共振光散射法测定脱氧核糖核酸 |
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| 引用本文： | 李玲,宋功武,方光荣,刘立明,信玲.孔雀石绿在核酸表面分子的长距组装及其共振光散射法测定脱氧核糖核酸[J].湖北大学学报(自然科学版),2002,24(3):246-249,271. |
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| 作者姓名： | 李玲 宋功武 方光荣 刘立明 信玲 |
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| 作者单位： | 湖北大学化学与材料科学学院，湖北武汉430062 |
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| 基金项目： | 中国分析测试基金(99-11) |
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| 摘 要： | 研究了在表面活性剂十六烷基三甲基溴化铵（CTMAB）的存在下孔雀石绿与DNA作用的共振光散射光谱。加入DNA后，在pH9.5-10.0范围内，400nm和482nm处出现增强的共振散射峰。考察了影响因素和最佳反应条件的选择，据此拟定了一种DNA的纳克级测定方法。此方法灵敏度高，干扰小，检测限低，实验结果令人满意。
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| 关 键 词： | 核酸表面分子 孔雀石绿 共振光散射法 脱氧核糖核酸 长距组装 含量测定 阳离子表面活性剂 纳克级 |
| 文章编号： | 1000-2375(2002)03-0246-04 |
Long range assembly of malachite green the molecular surfaces of nucleic acid and the determination of nucleic acid by resonance linght scattering technique |
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| Li Ling,Song Gongwu,Fang Gangrong,Liu Liming,Xin Ling.Long range assembly of malachite green the molecular surfaces of nucleic acid and the determination of nucleic acid by resonance linght scattering technique[J].Journal of Hubei University(Natural Science Edition),2002,24(3):246-249,271. |
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| Authors: | Li Ling Song Gongwu Fang Gangrong Liu Liming Xin Ling |
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| Abstract: | Resonance light - scattering characteristics of interaction between malachite green with ctDNA was studied at the present of cetyltrimethylammonium bromide. In the pH range of 9.5 - 10.0, malachite green exhibited a maximum of absorption at 400 ran and 482 nm when ctDNA was added. The effects of the determination of procedure were investigated and a new method for the determination of DNA at nanogram level based on RLS measurement was established with high sensitivity and selectivity. |
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| Keywords: | malachite green resonance light- scattering technique nucleic acid long range assembly |
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