| 纳豆激酶基因导入番茄的研究 |
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| 引用本文： | 袁琳,刘红海,王吟,李晓翔,杨艳燕.纳豆激酶基因导入番茄的研究[J].湖北大学学报(自然科学版),2006,28(2):181-182,186. |
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| 作者姓名： | 袁琳 刘红海 王吟 李晓翔 杨艳燕 |
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| 作者单位： | 湖北大学生命科学学院 湖北武汉430062 |
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| 基金项目： | 湖北省自然科学基金
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湖北省重点实验室基金 |
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| 摘 要： | 将来源于枯草芽孢杆菌的纳豆激酶(nattokinase,NK)基因转化到人类可以直接食用的,又非常喜爱的蔬菜—番茄中,得到在转录水平表达的转基因植株.通过根癌农杆菌EHA105介导,转化番茄的下胚轴,诱导愈伤组织形成及分化成幼苗,进而再生出完整植株.提取再生植株基因组DNA,通过PCR扩增检测,结果表明纳豆激酶基因已经成功整合到番茄植物基因组中.RT-PCR实验结果证明了纳豆激酶基因在转录水平上有表达.
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| 关 键 词： | 纳豆激酶基因 番茄 遗传转化 根癌农杆菌 |
| 文章编号： | 1000-2375（2006）02-0181-02 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005-09-26 |
Transformation of NK genes into tomato |
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| YUAN Lin,LIU Hong-hai,WANG Yin,LI Xiao-xiang,YANG Yan-yan.Transformation of NK genes into tomato[J].Journal of Hubei University(Natural Science Edition),2006,28(2):181-182,186. |
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| Authors: | YUAN Lin LIU Hong-hai WANG Yin LI Xiao-xiang YANG Yan-yan |
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| Institution: | School of Life Science, Hubei University, Wuhan 430062, China |
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| Abstract: | Nattokinase(NK) is a fibrinolytic enzyme that is extracted from natto.In fact it has enzymatic property of protease.So nattokinase can be developed as a new frbrinolytic medicine.In this research,tomato plants will be transformed to express nattokinase gene.Hypocotyle were cut from 'xifen 3'(Lycopersicon esculentum),inoculated with Agrobacterium tumefaciens EHA105 containing nk gene.Plants were regenerated on selective media with Kanamysin.The presence of the nk gene in tomato's genome was confirmed by PCR in plants surviving sections.RT-PCR results showed that nk gene have bene expressed at the transcripition level.And this result haven't bene reported in China so far. |
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| Keywords: | nattokinase gene tomato genetic transformation Agrobacterium tumefaciens |
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