绿色荧光蛋白在分子细胞生物学研究中的应用

绿我荧光蛋白具有优良的特性，在蓝光或长紫外光的激发下，不需要任何外源底物或内源辅助因子的参入就能发出绿色荧光，绿色荧光蛋白基因的表达可用来监控活细胞或生物体中基因表达和蛋白质的定位，这是一个革命性的进展，而且，对基因ＤＮＡ序列的改造可能使绿色荧光蛋白的发光特性更加优良，从而共应用范围会更加广泛。

The Use of Green Fluorescent Protein in the Study of Molecular Cell BioIogy

Green fluorescent protein has several good characters. Under excitation of long UV light or bluelight, it emits gi'een fluorescence without requiring any exogenous substlates and cofactors. GFP gene expression canbe used to monitor gene expression and protein localization in living cells and organisms. This is a development ofrevolutionaly sighficance. The DNA seqUence of this gene can be re - eopneered by mubenesis and the or willget stroved fluorescent propelties. The applications of GFP will be wider and wider.