| 荧光光度法研究杯[6]芳烃与蛋白质的相互作用 |
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| 引用本文： | 闫秋君,何瑜,宋功武.荧光光度法研究杯[6]芳烃与蛋白质的相互作用[J].湖北大学学报(自然科学版),2006,28(1):60-62. |
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| 作者姓名： | 闫秋君 何瑜 宋功武 |
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| 作者单位： | 湖北大学化学与材料科学学院 湖北武汉430062 |
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| 基金项目： | 湖北省教育厅科研项目
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湖北省重点实验室基金 |
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| 摘 要： | 用荧光光度法研究了杯6]芳烃磺酸钠与蛋白质的相互作用.求出了杯6]芳烃磺酸钠与蛋白质作用的形成常数,络合比和结合位点数.结果表明,杯6]芳烃磺酸钠在一定条件下能静态猝灭蛋白质的荧光,同时初步探讨了体系相互作用力.
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| 关 键 词： | 牛血清蛋白(BSA) 杯[6]芳烃磺酸钠 杯芳烃 荧光光度法 |
| 文章编号： | 1000-2375(2006)01-0060-03 |
| 收稿时间： | 04 1 2005 12:00AM |
| 修稿时间： | 2005年4月1日 |
Interaction of calix[6]arene and protein by spectral methods |
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| YAN Qiu-jun,HE Yu,SONG Gong-wu.Interaction of calix[6]arene and protein by spectral methods[J].Journal of Hubei University(Natural Science Edition),2006,28(1):60-62. |
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| Authors: | YAN Qiu-jun HE Yu SONG Gong-wu |
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| Institution: | School of Chemistry and Material Science, Hubei University, wuhan 430062, China |
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| Abstract: | The interactions of p-sulfonic sodium calixarene and BSA were studied by the methods of fluorescence.The compositions and resultant constants of the BSA-calix6]arene system were determined by fluorescence method.Calix6]arene can quench the fluorescence intensity of BSA by static quenching style.The optimum reaction conditions and the way of interaction between Calix6]arene and BSA were discussed. |
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| Keywords: | BSA(bovine serum albumin) p-sulfonated sodium of calix[6]arene calixarene fluorescence method |
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