超滤膜分离纯化山麦冬多糖的研究

采用不同孔径的超滤膜对山麦冬多糖提取液进行超滤分离,并用苯酚-硫酸比色法和福林-酚试剂显色法分别测定各超滤液多糖和蛋白的含量.结果表明不同相对分子量范围的多糖在山麦冬总糖中的含量分别为:相对分子量在30 000以上的含量为50.3%,30 000~10 000之间的含量为19.6%,10 000~1 000之间的含量为13.8%,分子量小于1 000的低聚糖和单糖含量为16.3%;各级多糖干物质纯度均大于90%;超滤膜可截留大部分蛋白质.超滤是一种很好的分离纯化山麦冬多糖的方法.

Isolation and purification of Radix Liriopes Polysaccharide by ultrafiltration membrane

Radix Liriopes Polysaccharide were ultrafiltrated by ultrafiltration membrane of different aperture,and the content of polysaccharide and protein of the different ultrafiltration process were determinated by phenol-sulfuric acid method and folin-phenol reagent method respectively.The result showed that the proportion of the polysaccharide with different molecular weight(MW) in the total polysaccharide were as follows:50.3?% for MW above 30?000,19.6?% for MW between 30?000 and 10?000,13.8?% for MW between 10?000 and 1?000,16.3?% for MW less than 1?000.The purification of the polysaccharide after the ultrafiltration process was over 90?%,most of protein can be eliminated.Ultrafiltration is a good method for the isolation and purification of Radix Liriopes Polysaccharide.