| 银杏叶片愈伤组织培养及光暗条件对黄酮含量的影响 |
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| 引用本文： | 秦公伟,曹小勇,张静,张威威,赵艳妮.银杏叶片愈伤组织培养及光暗条件对黄酮含量的影响[J].陕西理工学院学报(自然科学版),2011,27(1):71-76. |
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| 作者姓名： | 秦公伟 曹小勇 张静 张威威 赵艳妮 |
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| 作者单位： | 1. 陕西理工学院生物科学与工程学院,陕西省资源生物重点实验室,陕西,汉中,723001
2. 陕西理工学院历史文化系,陕西,汉中,723001 |
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| 基金项目： | 陕西省教育厅科研基金，陕西理工学院科研基金 |
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| 摘 要： | 以银杏嫩叶为外植体,在添加不同种类和浓度激素的MS培养基上进行愈伤组织诱导研究,同时分析光暗条件对愈伤组织生长和黄酮含量的影响。愈伤组织生长后,用分光光度比色法分析测定银杏愈伤组织黄酮含量。实验结果:在光照条件下,添加激素6-BA1.0mg/L和NAA2.0 mg/L的MS培养基中,诱导形成的愈伤组织生长最好,黄酮含量较高。
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| 关 键 词： | 银杏 愈伤组织 光暗条件 黄酮含量 |
Callus culture from young leaves of Ginkgo biloba.L and flavonoids content in the calli cultured in light or dark conditions |
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| QIN Gong-wei,CAO Xiao-yong,ZHANG Jing,ZHANG Wei-wei,ZHAO Yan-ni.Callus culture from young leaves of Ginkgo biloba.L and flavonoids content in the calli cultured in light or dark conditions[J].Journal of Shananxi University of Technology:Natural Science Edition,2011,27(1):71-76. |
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| Authors: | QIN Gong-wei CAO Xiao-yong ZHANG Jing ZHANG Wei-wei ZHAO Yan-ni |
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| Institution: | QIN Gong-wei1,CAO Xiao-yong1,ZHANG Jing2,ZHANG Wei-wei1,ZHAO Yan-ni1(1.School of Biological Sciences & Engineering,Shaanxi University of Technology,Hanzhong 723001,China,2.Department of History & Culture,China) |
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| Abstract: | Young leaves of Ginkgo biloba.L as explants cultured on MS medium containing different types and concentrations of hormones were for callus induction,and the effect of light on the growth of callus and content of flavonoids were also considered;After the callus proliferation,content of flavonoids in the calli was determinated by spectrophotometer in order to screen the best medium for the growth of callus with the highest flavonoid content.The results showed that in the light conditions,callus induced on MS... |
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| Keywords: | Ginkgo biloba L callus light or dark conditions flavonoids content |
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