| 竹黄菌丝体中竹红菌甲素的提取工艺 |
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| 引用本文： | 杜文,孙春龙,韩燕峰,梁建东,董旋,梁宗琦,郁建平,郭丽丽,张红涛.竹黄菌丝体中竹红菌甲素的提取工艺[J].贵州大学学报(自然科学版),2010,27(6):70-73. |
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| 作者姓名： | 杜文 孙春龙 韩燕峰 梁建东 董旋 梁宗琦 郁建平 郭丽丽 张红涛 |
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| 作者单位： | [1]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025 [2]贵州大学农业生物工程省重点实验室教育部绿色农药与农业生物工程重点实验室,贵州贵阳550025 [3]贵州大学贵州省生化工程中心,贵州贵阳550025 [4]山东省临沂市兰山区农业局,山东临沂276000 [5]青岛市市立医院,山东青岛266011 |
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| 基金项目： | 贵州省自然科学基金项目，贵州大学引进人才科研项目，贵州大学生命科学学院研究生创新基金 |
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| 摘 要： | 采用浸提法对竹黄菌液体发酵竹红菌甲素的提取工艺进行优化,以竹红菌甲素得率为评价指标,选用单因素实验和正交试验,考察有机溶剂、粉碎与否、提取物干燥温度、浸提温度、有机溶剂的量、浸提时间等因素对竹黄菌丝体竹红菌甲素得率的影响。实验结果表明：对3.0g的菌丝体采用35℃干燥温度,粉碎后加入无水乙醇的量为40mL,浸提温度为30℃,浸提时间24h,可以获得最佳的竹红菌甲素浸提效果。
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| 关 键 词： | 竹黄菌 竹红菌甲素 提取工艺 |
Study on Extraction Technology of Hypocrellin A from the mycelia of Shiraia bambusicola |
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| DU Wen,SUN Chun-long,HAN Yan-feng,LIANG Jian-dong,DONG Xuan,LIANG Zong-qi,YU Jian-ping,GUO Li-li,ZHANG Hong-tao.Study on Extraction Technology of Hypocrellin A from the mycelia of Shiraia bambusicola[J].Journal of Guizhou University(Natural Science),2010,27(6):70-73. |
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| Authors: | DU Wen SUN Chun-long HAN Yan-feng LIANG Jian-dong DONG Xuan LIANG Zong-qi YU Jian-ping GUO Li-li ZHANG Hong-tao |
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| Institution: | 1.Institute of Fungus Resources,Guizhou University,Guiyang 550025,China;2.Guizhou Key Laboratory of Agricultural Bioengineering,Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education Guizhou University,Guiyang 550025,China;3.Center of Biochemical Engineering in Guizhou Province,Guizhou University,Guiyang 550025,China;4.Agricultural Bureau of Lanshan District Lingi City Shandong Provience,Linyi 276000,China;5.Qingdao Municipal Hospital,Qingdao 266011,China) |
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| Abstract: | Experiments were designed to study the technical condition of ethanol extraction from mycelia of Shiraia bambusicola.The optimal process was studied with yield and content of Hypocrellin A by single factor tests and orthogonal test to show the effects of temperature,time,ratio of materials,organic solvents,smashing level and extract drying temperature.The experimental results show that the optimum technological conditions were,mycelia on 3.0 g with drying temperature 35℃,after smashing the amount of ethanol added was 40 ml,the total time 24 h,extraction temperature of 30℃,extraction time 24 h,the best Hypocrellin A extraction results was obtained. |
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| Keywords: | shiraia bambusicola hypocrellin A extraction technology |
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