| 一株拮抗纹枯病菌放线菌的筛选及鉴定 |
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| 引用本文： | 崔宇辉,王媛媛,汪鹏荣,高爱同,曹丽凌,蒋冬花.一株拮抗纹枯病菌放线菌的筛选及鉴定[J].浙江师范大学学报(自然科学版),2012(4):441-445. |
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| 作者姓名： | 崔宇辉 王媛媛 汪鹏荣 高爱同 曹丽凌 蒋冬花 |
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| 作者单位： | 浙江师范大学化学与生命科学学院 |
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| 基金项目： | 浙江省公益性应用研究计划项目(2010C32076) |
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| 摘 要： | 从采自金华市各地的68份土样中分离得到了放线菌406株，以水稻纹枯病菌为指示菌，利用平皿对峙法筛选到42株具有较好拮抗作用的放线菌菌株．其中，Sh-43菌株的拮抗作用最强，抑菌带宽度为28．3mm．根据培养特征、形态特征、生理生化特征及16S rDNA序列比对分析，鉴定Sh-43菌株为吸水链霉菌（Streptomyces hygroscopicus）．
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| 关 键 词： | 纹枯病菌 放线菌 拮抗作用 筛选 鉴定 |
Screening and identification of antagonistic actinomycete against Rhizoctonia solani |
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| CUI Yuhui,WANG Yuanyuan,WANG Pengrong,GAO Aitong,CAO Lilin,JIANG Donghua.Screening and identification of antagonistic actinomycete against Rhizoctonia solani[J].Journal of Zhejiang Normal University Natural Sciences,2012(4):441-445. |
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| Authors: | CUI Yuhui WANG Yuanyuan WANG Pengrong GAO Aitong CAO Lilin JIANG Donghua |
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| Institution: | (College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China) |
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| Abstract: | Four hundred and six actinomycetes were isolated from sixty eight soil samples which were collected from different areas of Jinhua. Forty-two strains had been proved to have antagonistic activity against Rhizocto- nia solani by confronting cultivation on PDA plates. Among them, the strain Sh-43 exhibited the strongest an- tibiosis capacity with an inhibition zone of 28.3 mm. The strain Sh-43 was identified as scopicus on the cultural, morphological, physiological, biochemical characteristics and 16S rDNA gene se- quence analysis. |
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| Keywords: | Rhizoctonia solani actinomycete antagonistic action screening identification |
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