| 乙醇注入法制备司帕沙星脂质体 |
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| 引用本文： | 丁丽燕,杨春,李学明,张慧颖,韦萍.乙醇注入法制备司帕沙星脂质体[J].南京工业大学学报(自然科学版),2007,29(1):32-35. |
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| 作者姓名： | 丁丽燕 杨春 李学明 张慧颖 韦萍 |
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| 作者单位： | 南京工业大学,制药与生命科学学院,江苏,南京,210009 |
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| 摘 要： | 制备司帕沙星脂质体,单因素考察优化了制备工艺和处方,以提高脂质体的包封率.采用乙醇注入高压均质法制备了司帕沙星脂质体,以透析高效液相色谱法测定了含量和包封率.实验结果表明,司帕沙星脂质体的平均粒径为22.1nm,包封率为47%左右.乙醇注入高压均质法适用于制备司帕沙星脂质体,透析高效液相色谱法操作简单、准确、重复性好,可用于测定司帕沙星脂质体的含量和包封率.
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| 关 键 词： | 司帕沙星脂质体 乙醇注入 包封率 单因素考察 乙醇注入 法制 司帕沙星 脂质体 ethanol injection liposome sparfloxacin 色谱法测定 操作 高效液相色谱法 高压均质法 平均粒径 结果 实验 含量 透析 包封率 处方 制备工艺 优化 |
| 文章编号： | 1671-7627(2007)01-0032-04 |
| 修稿时间： | 2006-09-25 |
Preparation of sparfloxacin liposome by ethanol injection |
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| DING Li-yan,YANG Chun,LI Xue-ming,ZHANG Hui-ying,WEI Ping.Preparation of sparfloxacin liposome by ethanol injection[J].Journal of Nanjing University of Technology,2007,29(1):32-35. |
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| Authors: | DING Li-yan YANG Chun LI Xue-ming ZHANG Hui-ying WEI Ping |
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| Institution: | College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China |
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| Abstract: | With the aim to prepare sparfloxacin liposome and to improve entrapment efficiency, the main factor affecting the quality of sparfloxacin liposome in the preparation was studied by single factor exploration. Sparfloxacin liposome was prepared by ethanol injection and high homogenizer methods. Sparfloxacin liposome and its entrapment efficiency were determined by dialysis-HPLC. The results revealed that the mean size of liposome was 22. 1 nm and entrapment efficiency was about 47%. It was viable to prepare liposome such as Sparfloxacin by ethanol injection and high homogenizer methods. Dialysis-HPLC method was convenient, accurate and suitable for the determination of Sparfloxacin and the entrapment efficiency in Sparfloxacin liposome. |
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| Keywords: | sparfloxacin ethanol injection entrapment efficiency single factor exploration |
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