彩色马蹄莲叶片愈伤组织培养体系的建立

采用彩色马蹄莲（Zantedeschia spp cv Pink Giant）的叶片为外植体，运用正交设计方法，对诱导马蹄莲愈伤组织培养基进行了预试验，并对影响马蹄莲愈伤组织诱导的因素进行了定量分析；在正交试验基础上，设计了14种不同激素种类和配比的配方，进行了诱导愈伤组织体系的建立．对愈伤组织发生过程中形态学和细胞学结构进行了动态观察．结果表明：在MS基本培养基中添加6-BA和2，4-D对以叶片外植体的愈伤诱导率有显著影响；对初代诱导的愈伤组织进行的两次继代培养结果表明，继代和愈伤组织继续发生的最佳培养基为MS附加2，4-D2.0mg／L和BA2，0mg／L．

Establishment of Callus Induction System from Leaves in Zantedeschia

By the orthogonal design the medium is tested for callus induction from leaf explants in calla lily. The effection of the plant growth regulators on initiation and culture of callus is confirmed. 14 selections of dif- ferent hormones and ratios for the medium are designed based on the orthogonal test, and the callus culture system is established. The morphorlogical and cytological structures are observed dynamically during callus in- duction. The results show that MS media supplemented with 6-BA and 2, 4-D is favorable to the induction ratio for leaf callus. The optimum media for callus subculture is MS media supplemented with 2, 4-D 2.0 mg/L and BA 2.0 mg/L or with NAA 2.0 mg/L and BA 2.0 mg/L.