| 蓝隐藻藻蓝蛋白的分离、纯化及性质研究 |
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| 引用本文： | 张允允,陈敏.蓝隐藻藻蓝蛋白的分离、纯化及性质研究[J].烟台大学学报(自然科学与工程版),2011(4). |
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| 作者姓名： | 张允允 陈敏 |
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| 作者单位： | 烟台大学化学生物理工学院; |
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| 基金项目： | 国家自然科学基金资助项目(40976083) |
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| 摘 要： | 以实验室培养的蓝隐藻(Chroomonas placoidea)为材料,经过压力破碎和高速离心分离去除膜成分,得到的上清液分别采用50%、70%、80%、90%和100%的硫酸铵分级沉淀.沉淀经2次葡聚糖Sephadex G-100层析,得到了纯化的藻蓝蛋白,并对各级藻蓝蛋白的吸收光谱、荧光谱、亚基组成进行了分析.结果显示,隐藻藻胆蛋白呈现出很宽的硫铵沉淀范围,但各种沉淀样品具有几乎相同的光谱特性和相似的亚基组成,说明其不同的水溶性质可能与其亚基聚合程度不同有关;得到的藻蓝蛋白经HPLC和中性PAGE检测,基本不含杂质,A645/A280比值最高可达7以上.结果为今后的研究和应用奠定了基础.
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| 关 键 词： | 蓝隐藻 藻蓝蛋白 分子筛 高效液相色谱 |
Isolation,Purification and Characterization of Phycocyanin from Chroomonas placiodea |
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| ZHANG Yun-yun,CHEN Min.Isolation,Purification and Characterization of Phycocyanin from Chroomonas placiodea[J].Journal of Yantai University(Natural Science and Engineering edirion),2011(4). |
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| Authors: | ZHANG Yun-yun CHEN Min |
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| Institution: | ZHANG Yun-yun,CHEN Min(School of Chemical and Biological Science and Engineering,Yantai University,Yantai 264005,China) |
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| Abstract: | Chroomonas placoidea cells cultured in laboratory are broken and centrifuged to remove the membrane debris.The supernatants are precipitated by 50%,70%,80%,90% and 100% of ammonium sulfate respectively.Sediments containing PC are purified by two steps of Sephadex G-100gel filtration chromatography.The absorption spectra,fluorescence spectroscopy,as well as subunit composition of purified PC are determined.The results show that the PC of C.placoidea precipitates during a quite broad range of ammonium sulfate... |
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| Keywords: | Chroomonas placoidea phycocyanin gel filtration chromatography HPLC |
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