| 超声波协同酶法提取浒苔多糖工艺的研究 |
| |
| 引用本文： | 唐志红,王硕,鞠宝,温少红,秦松.超声波协同酶法提取浒苔多糖工艺的研究[J].烟台大学学报(自然科学与工程版),2013,26(4):303-306. |
| |
| 作者姓名： | 唐志红 王硕 鞠宝 温少红 秦松 |
| |
| 作者单位： | 1. 烟台大学生命科学学院,山东烟台,264005
2. 中国科学院烟台海岸带研究所,山东烟台,264003 |
| |
| 基金项目： | 山东省高等学校科技计划项目 |
| |
| 摘 要： | 以浒苔为原料,采用超声波协同酶法提取多糖.在单因素试验的基础上采用正交试验优化浒苔多糖提取的工艺条件,并与热水浸提法进行比较.结果表明,浒苔多糖提取的最佳条件:超声时间20 min,料液比1∶50,温度50℃,酶用量3.0%,在此条件下重复试验,测得浒苔多糖的平均提取率为16.83%,同热水浸提法相比,多糖提取率明显提高,且提取时间大大缩短.
|
| 关 键 词： | 超声波 纤维素酶 浒苔 多糖 |
Extraction Process of Polysaccharides from Enteromorpha prolifera by Ultrasonic-Assisted Enzymatic Methods |
| |
| TANG Zhi-hong
,
WANG Shuo
,
JU Bao
,
WEN Shao-hong
,
QIN Song.Extraction Process of Polysaccharides from Enteromorpha prolifera by Ultrasonic-Assisted Enzymatic Methods[J].Journal of Yantai University(Natural Science and Engineering edirion),2013,26(4):303-306. |
| |
| Authors: | TANG Zhi-hong WANG Shuo JU Bao WEN Shao-hong QIN Song |
| |
| Institution: | 2 ( 1. College of Life Science, Yantai University, Yantai 264005, China; 2. Yantai Institute of Coastal Zone Research, Chinese Acade- my of Sciences, Yantai 264003, China) |
| |
| Abstract: | Ultrasonic-assisted enzymatic methods are adopted to extract polysaccharides from Enteromorpha prolif- era. Based on single factor experiment results, orthogonal test L9 （34） is applied to optimize the extraction condi- tions. The results show that the optimum extraction conditions are as follows ： ultrasonic time 20 min, ratio of mate- rial to water 1：50, hydrolysis temperature 50 ℃, enzyme concentration 3%. Under the optimized conditions, the extraction rate reaches 16. 83%, which is higher than that of traditional hot water extraction, and the extraction time is significantly reduced. The optimized conditions can be used for the extraction of Enteromorpha prolifera pol- ysaccharides. |
| |
| Keywords: | ultrasonic cellulase Enteromorpha proliferae polysaccharides |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
