|
|||||
|
|
| 弹性蛋白酶抑制剂筛选方法的建立及天然产物对其抑制活性观察 | |
| 引用本文： | 陈立平,尹相利,方玉春,郝立杰,赵烽.弹性蛋白酶抑制剂筛选方法的建立及天然产物对其抑制活性观察[J].烟台大学学报(自然科学与工程版),2011,24(3). |
| 作者姓名： | 陈立平 尹相利 方玉春 郝立杰 赵烽 |
| 作者单位： | 1. 烟台大学药学院,山东烟台,264005 2. 海洋药物教育部(中国海洋大学)重点实验室,山东青岛,266003 3. 烟台赛尔斯生物技术有限公司,山东烟台,264006 |
| 基金项目： | 海洋药物教育部重点实验室(中国海洋大学)开放基金资助项目 |
| 摘 要： | 发现弹性蛋白酶抑制剂是治疗肺气肿等疾病药物的重要研发方向之一.本研究用弹性蛋白酶建立微量、高效的体外抑制剂筛选模型,利用西维来司钠作为阳性对照,验证了筛选模型的稳定性和灵敏度,并筛选了33种中药单体对弹性蛋白酶的抑制活性,其中部分化合物表现一定的抑制活性. |
| 关 键 词： | 弹性蛋白酶 筛选方法 活性筛选 |
Establishment of Assay for Screening of Elastase Inhibitor and Inhibitory Effect of Natural Products on Activity of Elastase |
|
| CHEN Li-ping,YI Xiang-li,FANG Yu-chun,HAO Li-jie,ZHAO Feng.Establishment of Assay for Screening of Elastase Inhibitor and Inhibitory Effect of Natural Products on Activity of Elastase[J].Journal of Yantai University(Natural Science and Engineering edirion),2011,24(3). | |
| Authors: | CHEN Li-ping YI Xiang-li FANG Yu-chun HAO Li-jie ZHAO Feng |
| Institution: | CHEN Li-ping1,YI Xiang-li1,FANG Yu-chun2,HAO Li-jie3,ZHAO Feng1(1.School of Pharmacy,Yantai University,Yantai 264005,China,2.Key Laboratory of Marine Drugs,Ocean University of China,Qingdao 266003,3.Yantai Science & Biotechnology Company Limited,Yantai 264006,China) |
| Abstract: | Discovery of elastase inhibitors has become much attractive in the field of drug research.In this study,we established an assay for screening new drugs by using purified elastase.Furthermore,for thirty-three natural products isolated from medicinal plants,we investigate their inhibitory activity of elastase.Some of them exhibite inhibitory effect on the activity of elastase,such as ursolic acid,rutin,hyperoside and tetrandrine. |
| Keywords: | elastase screening method natural product |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |