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仙客来(Cyclaman persicum,Mill)脱分化过程中核酸含量动态研究
引用本文:李丽霞,曲复宁,赵吉强,由翠荣,付学军,王丽.仙客来(Cyclaman persicum,Mill)脱分化过程中核酸含量动态研究[J].烟台大学学报(自然科学与工程版),2004,17(1):38-41.
作者姓名:李丽霞  曲复宁  赵吉强  由翠荣  付学军  王丽
作者单位:烟台大学,生物化学系,山东,烟台,264005
基金项目:山东省科技厅科技攻关项目资助(012010121).
摘    要:研究了仙客来叶柄和球茎脱分化过程中核酸的动态变化。结果表明,培养后2d,DNA和RNA含量增加迅速,出现峰值,培养过程中RNA含量波动式上升,变化幅度大于DNA,RNA/DNA增加,DNA代谢相对较稳定;总核酸含量表现为稳中有升的趋势,叶柄和球茎的核酸代谢特征无明显差异。

关 键 词:仙客来  脱分化  核酸代谢  组织培养  核酸含量
文章编号:1004-8820(2004)01-0038-04

Study on Continuous Change of Nucleic Acid in the Process of Dedifferentiation of Cyclamen
LI Li-xia,QU Fu-ning,ZHAO Ji-qiang,YOU Cui-rong,FU Xue-jun,WANG Li.Study on Continuous Change of Nucleic Acid in the Process of Dedifferentiation of Cyclamen[J].Journal of Yantai University(Natural Science and Engineering edirion),2004,17(1):38-41.
Authors:LI Li-xia  QU Fu-ning  ZHAO Ji-qiang  YOU Cui-rong  FU Xue-jun  WANG Li
Abstract:The continuous change of nucleic acid in the process of dedifferentiation of cyclamen stalk and bulb are studied. The results show that in the third day after cultivation, the contents of DNA and RNA increase rapidly and peak value appears. During the cultivation, RNA's content grows wavily and the ratio of RNA/ DNA rises. The content of RNA changes by a bigger range than that of DNA, that is DNA metabolism remains stable. The total nucleic acid grows slowly. Metabolism character between stalk and bulb in cyclamen haven't obvious difference.
Keywords:cyclamen  dedifferentiation  nucleic acid metabolism
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